MOLECULAR TEST SYSTEMS IN VITRO IN EVALUATION OF THE PHARMACOLOGICAL ACTIVITY OF PLANT CELL BIOMASS

DOI: https://doi.org/10.29296/25877313-2018-01-01
Issue: 
1
Year: 
2018

V.A. Dubinskaya Ph.D. (Biol.), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: va@dubinsky.info V.A. Bykov Academician of RAS, Chief Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

Native herbs and cellular cultures, which were grown based on them and are in dedifferentiated state, often differ in synthesis of secondary metabolites, that causes a decrease or complete disappearance of the major active ingredients distinctive for native herbs. However, changing the metabolome of cellular cultures does not always mean the absence of the desired pharmacological activities in-herent to a native herb. Cells of a transplanted culture could accumulate not less active isomers or different metabolites with the same direction of pharmacologic effect. The molecular test systems in vitro allows to detect the presence of active substances and to under-stand the difference between cellular and native bioregulation of herbs.

Keywords: 
plant cellular cultures
Panax
Rhodiola rosea
Ungernia victoris
Arnica montana
test systems in vitro
glutation reductase
catalase

References: 
  1. Plant metabolomics. Methods and protocols / N.W. Hardy,
  2. R.D. Hall (Ed.) 2012. Humana Press. 340 p.
  3. Patent № 1735756 (RF). Shtamm kul'tiviruemyh kletok
  4. rastenij Rhodiola rosea – produtsent fenol'nyh soedine-
  5. nij adaptogenov / I.V. Aleksandrova, A.N. Danilina. 1990.
  6. Veutler E. Red cell metabolism / Ed. E. Beutler, C. Livingsone.
  7. 1986. P. 69–71.
  8. Koroljuk M.A., Ivanova L.I., Majorova I.G., Tokareva
  9. V.E. // Laboratornoe delo. 1988. № 1. S. 16–19.
  10. Collagete S.M., Molyneax R.J. Bioactive natural products.
  11. Detection, isolation and structural determination. Second
  12. edition. CRC Press, Taybor Francis Group. 2007. 624 p.
  13. Kurkin V.A., Zapesochnaja G.G., Dubicheva A.G., Vorontsov
  14. E.D., Aleksandrova I.V., Panova R.V. Fenilpropanoidy
  15. kallusnoj kul'tury Rhodiola rosea // Himija prirodnyh
  16. soedinenij. 1991. № 4. S. 481–490.