PLANT GROWTH REGULATORS INFLUENCE ON THE INDUCTION AND PROLIFERATION OF CALLUS HEDYSARUM ALPINUM L

DOI: https://doi.org/10.29296/25877313-2018-01-09
Download full text PDF
Issue: 
1
Year: 
2018

P.S. Savin Ph.D. (Biol.), Leading Research Scientist, All-Russian Research Scientific Institute of Medicinal and Aromatic Plants (Moscow) E-mail: savin-pavel@list.ru M.V. Savenkova Post-graduate Student, All-Russian Research Scientific Institute of Medicinal and Aromatic Plants (Moscow) T.A. Savina Ph.D. (Biol.), Head of Department of Biotechnology, All-Russian Research Scientific Institute of Medicinal and Aromatic Plants (Moscow) S.B. Myasnikova Research Scientist, All-Russian Research Scientific Institute of Medicinal and Aromatic Plants (Moscow)

The use of plant tissues cultivated on artificial nutrient media as producers of biologically active substances based on their abil-ity to synthesize biologically active compounds, characteristic of the whole plant. One of such medicinal plants is Hedysarum alpinum L. grass which is allocated xantonova glycoside mangiferin with high biological activity. In medicine use the herb Hedysarum for the production of the drug alpizarin. The purpose of this study was to obtain experimental data on the introduction into a sterile culture initiation of callus from na-tive plants Hedysarum alpinum L. The task was to identify, based on the type of Explant and plant hormone. Have studied the effect of growth regulators auxin, cytokinin nature and gibberellins on the callusogenesis from different parts of the plant Hedysarum Alpine. It was found that to obtain the primary callus and its further cultivation is required in a nutrient medium to make the auxins 2,4-D (0.1 mg/l); NAA (0.1 mg/l) and cytokinin BAP (1.0 mg/l). Best explants for obtaining callus tissue was cotyledons of seedling plants derived from the seed of intact plants.

Keywords: 
Sweetvetch Alpine
explant
callus
mangiferin

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.

References: 
  1. Vasil'eva I.S., Paseshnichenko V.A. Steroidnye glikozidy rastenij i kul'tury kletok dioskorei, ihmetabolizm i biologicheskaja aktivnost' // Uspehi biologicheskoj himii. 2000. T. 4. S. 153−204.
  2. Nosov A.M. Lekarstvennye rastenija. M.: Eksmo. 2004. 350 s. 3.
  3. Matkowski A., Kuś P., Góralska E., Woźniak D. Mangiferin a bioactive xanthonoid, not only from mango and not just antioxidant // Mini. Rev. Med. Chem. 2013 Mar. V. 13, № 3 R. 439−455.
  4. Vichkanova S.A. Effektivnost' alpizarina pri gerpesvirusnyh zabolevanijah u detej i vzroslyh // Prakticheskaja fitoterapija. 2000. № 1. S. 34−39.
  5. Zinner N.S. Biologicheskie osobennosti Hedysarum alpinum L. i Hedysarum theinum Krasnob. pri introduktsii v uslovijah lesnoj zony Zapadnoj Sibiri: Avtoref.dis. ... kand. biol. nauk. Tomsk. 2011. 19 c.
  6. Hotsialova L.I. Introduktsija lekarstvennogo rastenija kopeechnika al'pijskogo v nechernozem'e: Avtoref. dis. … kand. biol. nauk. M. 1989. 24 s.
  7. Bushkovskaja L.M., Pushkina G.P., Klimahin G.I. Prime nenie mikroelementov v sisteme zaschity lekarstvennyh kul'tur ot vreditelej // OOO AGRO HHI «Izdatel'stvo Agrorus». 2007. № 10−12. S. 38−39.
  8. Kuznetsova E.I., Dytskova E.V., Zhuchenko A.A. Ekologicheski bezopasnaja tehnologija vozdelyvanija kopeechnika al'pijskogo // Vestnik RGAZU. 2008. № 1. S. 36−37.
  9. Butenko R.G. Biologija kletok vysshih rastenij in vitro i biotehnologii na ih osnove. M.: FBK-PRESS. 1999. 159 s.