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Z.K. Nikitina Dr.Sc. (Biol), Professor, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: I.K. Gordonova Ph.D. (Biol), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

Cellulose is the most abundant biopolymer on the earth, which holds a special place in the cycle of organic carbon. The hydrolysis of this polysaccharide occurs under the action of cellulase, which are produced by many microorganisms: micromycetes, aerobic and anaerobic bacteria. The intensity of cellulases studies increased sharply in the 1970s which was due with the energy crisis. The shortage of nonrenewable sources of energy and materials is observed at present time too. Cellulose is an almost inexhaustible source of renewable raw materials, which can be converted enzymatically into glucose for further production of biofuel, amino acids, feed protein, etc. The main aim of this work was to develop the methodological approaches for selection of micromycetes with some cellulose activity. By means of surface cultivation on media with different types of cellulose the growth characteristics of 6 strains of micromycetes from the collection of VILAR were evaluated. It is established that all strains grew on all media containing cellulose. The peculiarities of the intensity and micromycetes character growth during different types of celluloses using were identified. The most intensive growth of micromycetes was observed on the medium with the Hyperici herba. Discovered zones of lysis on the media with the Hyperici herba during A. flavus studying allows to regard this micromycete as one of the most promising producer of cellulase. New strain of P. martensii adapted to the cellulose was obtained, and it was also promising as cellulase producer.


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  1. Skomarovskij A.A., Markov A.V., Gusakov A.V. Novye tselljulazy dlja vysokoeffektivnogo gidroliza lignotselljulaznoj biomassy // Prikladnaja biohimija i mikrobiologija. 2006. T. 42. № 6. R. 674−680.
  2. Rabinovich M.L., Bolobova A.V., Kondraschenko V.I. Teoreticheskie osnovy biotehnologii drevesnyh kompozitov. Drevesina i razlagajuschie ee griby. M.: Nauka. 2001. 264 s.
  3. Rabinovich M. L., Mel'nik M.S. Progress v izuchenii tselljuloliticheskih fermentov i mehanizm biodegradatsii vysokouporjadochennyh form tselljulozy // Uspehi biologicheskoj himii. 2000. № 40. S. 205−266.
  4. Chekushina A.V. Tselljuloliticheskie fermentnye preparaty na osnove gribov Trichoderma, Penicillium i Myceliophtora s uvelichennoj gidroliticheskoj aktivnost'ju: Avtoref. diss. … kand. him. nauk. M. 23 s.
  5. Chekushina A.V., Dotsenko G.S., Kondrat'eva E.G., Sinitsyn A.P. Komponentnyj sostav kommercheskih fermentnyh preparatov, poluchennyh s pomosch'ju gribov roda Trichoderma i prednaznachennyh dlja biokonversii rastitel'nogo syr'ja // Biotehnologija. 2013. № 3. S. 58−68.
  6. Chekushina A.V., Dotsenko G.S., Kondrat'eva E.G., Sinitsyn A.P. Fermentnye preparaty Penicillium verruculosum dlja biokonversii rastitel'nogo syr'ja – al'ternativa kommercheskim preparatam, poluchennyh s pomosch'ju gribov roda Trichoderma // Biotehnologija. 2013. № 3. S. 69−80.
  7. Rabinovich M.L., Mel'nik M.S., Bolobova A.V. Struktura i mehanizm dejstvija tselljuloliticheskih fermentov // Biohimija. 2002. T. 67. № 8. S. 1026−1050.
  8. Rabinovich M.L., Mel'nik M.S., Bolobova A.V. Tselljulazy mikroorganizmov // Prikladnaja biohimicheskaja mikrobiologija. 2002. T. 38. № 4. S. 355−373.
  9. Nikitina Z.K., Gordonova I.K. Vydelenie, ochistka i biohimicheskie svojstva keratinoliticheskogo fermenta, sekretiruemogo Penicillium citrinum // Voprosy biologicheskoj, meditsinskoj i farmatsevticheskoj himii. 2013. № 1. S. 36−41.
  10. Suhosyrova E.A., Nikitina Z.K., Jakovleva M.B. Vydelenie, ochistka i nekotorye svojstva kollagenoliticheskih fermentov, sekretiruemyh Aspergillus flavus // Voprosy biologicheskoj, meditsinskoj i farmatsevticheskoj himii. 2008. № 2. S. 46−49.
  11. Nikitina Z.K., Jakovleva M.B., Gordonova I.K., Zon Hy Chol. Sravnitel'naja otsenka rosta dejteromitsetov pri ispol'zovanii razlichnyh beloksoderzhaschih substratov // Voprosy biologicheskoj, meditsinskoj i farmatsevti cheskoj himii. 2015. № 11. S. 56−59.