THE DEVELOPMENT OF THE METHODICAL APPROACHES TO SEARCH FOR PRODUCERS OF CELLULASE

Cellulose is the most abundant biopolymer on the earth, which holds a special place in the cycle of organic carbon. The hydrolysis of this polysaccharide occurs under the action of cellulase, which are produced by many microorganisms: micromycetes, aerobic and anaerobic bacteria. The intensity of cellulases studies increased sharply in the 1970s which was due with the energy crisis. The shortage of nonrenewable sources of energy and materials is observed at present time too. Cellulose is an almost inexhaustible source of renewable raw materials, which can be converted enzymatically into glucose for further production of biofuel, amino acids, feed protein, etc. The main aim of this work was to develop the methodological approaches for selection of micromycetes with some cellulose activity. By means of surface cultivation on media with different types of cellulose the growth characteristics of 6 strains of micromycetes from the collection of VILAR were evaluated. It is established that all strains grew on all media containing cellulose. The peculiarities of the intensity and micromycetes character growth during different types of celluloses using were identified. The most intensive growth of micromycetes was observed on the medium with the Hyperici herba. Discovered zones of lysis on the media with the Hyperici herba during A. flavus studying allows to regard this micromycete as one of the most promising producer of cellulase. New strain of P. martensii adapted to the cellulose was obtained, and it was also promising as cellulase producer.