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THE DEVELOPMENT AND VALIDATION OF THE ARBUTIN DETERMINATION PROCEDURE IN THE BEARBERRY LEAVES BY HPLC/МS

DOI: https://doi.org/10.29296/25877313-2018-05-01
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Issue: 
5
Year: 
2018

A.V. Nikulin Ph.D. (Chem.), Shared Research and Education Center of the Peoples’ Friendship University of Russia (Moscow) M.V. Okuneva Dr.Sc. (Pharm.), Research and Education Center of the Peoples’ Friendship University of Russia (Moscow) S.V. Goryainov Dr.Sc. (Pharm.), Research and Education Center of the Peoples’ Friendship University of Russia (Moscow) O.G. Potanina Dr.Sc. (Pharm.), Research and Education Center of the Peoples’ Friendship University of Russia (Moscow) E-mail: R251@yandex.ru

In the article the arbutin determination procedure in the bearberry's leaves is offered. As the method of determination modern, sufficciently sensitive, and effective method HPLC/MS was chosen. The conditions were selected, the main metrological characteristics were estimated. Mass-spectrometric conditions for determination of arbutin at mode multiple reactions monitoring (MRM) were internal standard (phenolphalein), fragmentor's voltage, energy of collision cell, precursor ion, product ion, drying gas temperature ‒ 320°C, pressure gas of nebulaizer – 40 psi, drying gas rate ‒ 10 l/min, capillar's voltage – 4 kV. Conditions of elution were selected: column Protecol C18 HPH125 (250x4,6 mm, 5 μm), gradient, elution rate. Thereby, the procedure of quantitative determination of arbutin the bearberry's leaves with HPLC/MS was devised. The basic metrological characterisctics of the developed procedure were estimated. It has been shown that HPLC/MS and the developed procedure have high efficiency and prospects and can be recommended for arbutin content determination in medicinal raw plants.
Keywords: 
bearberry leaves
arbutin
HPLC/MS
validation of procedure

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