ANTI-INFLAMMATORY ACTIVITY OF HUMIC ACIDS OF PELOIDS

This work is dedicated to the research of the anti-inflammatory activity of humic acids of peloids within the model of acute carrageenin inflamma-tion. The object of the study is a drug based on humic acids, isolated from low-mineralized silt mud. The experiment was carried out on four groups, each consisting of 20 laboratory animals. In the first group, rats received an intramuscular physio-logical solution with a volume of 0.2 cm3. In the second group, rats were injected with a 0.10 % solution of the humic acid drug in a dose of 0.2 cm3. In the third group, 0.1 cm3 of a 1.0% solution of carrageenin was subcutaneously injected in the right hind paw. In the fourth group, the animals received 0.2 cm3 of a 1.0% solution of carrageenin one time after a preventive intramuscular injection of a 0.2 cm3 0.10% aqueous solution of humic acids. Blood for analysis was taken after decapitation 3 hours after injection and the blood composition was determined according to conventional methods. The introduction of humic acids simultaneously with carrageenin neutralizes changes in the parameters of peripheral blood and leads to less pro-nounced leukocytosis, which is observed with carrageenin edema. The number of leukocytes in the fourth group of rats is reduced by 57%, ESR (Erythro-cyte Sedimentation Rate) by 68%, neutrophilic granulocytes by 34% in relation to the third group. The preventive administration of humic acids showed a lack of C-reactive protein in the serum, as well as a decrease in the values of spontaneous and stimulated chemo-luminescence of leukocytes by 54% and 33%, respectively. Interleukin 1-β level decreased by 39% when treated with the drug. The results of the analysis of the qualitative and quantitative composition of blood and its elemental elements according to 19 quantitative indices show that the administration of the drug reduces the number of neutrophilic granulocytes, inhibits the excessive formation of interleukin 1-β. The data of the leukocyte blood formula of animals of the 3rd and 4th groups indicate that the use of the drug in the development of inflammation significantly reduces the degree of deviation of the blood values from the control values. Thus, the results of the study of the anti-inflammatory activity of humic acids show the effectiveness of their use during the acute phase of carra-geenin inflammation. Humic acids of peloids are a promising natural substance for the creation of new products characterized by a high pharmacological effect with minimal side effects.