I.B. Pavlova Research Scientist, Lomonosov Moscow State University E-mail: T.S. Sharkova Ph.D. (Biol.), Lomonosov Moscow State University L.V. Podorolskayay Ph.D. (Biol.), Lomonosov Moscow State University

We studied the interaction of two antithrombin preparations: experimental complex of proteinases Longolytin from an anamorphic fungus Ar-throbotrys longa (rectal application) and commercial preparation Pijavitum from the medical leech Hirudo medicinalis (oral administration) in experiments in vivo on 44 mongrel rats in for 6 days. The data were compared with the results obtained in the individual usage of these drugs. One of the objectives of our the study was to increase the effectiveness of Longolitin as a thrombolytic and antithrombotic drug by adding to it synergistic substances. Among them, we chose to study the Russian drug Piyavit, which has antithrombotic and anticoagulant properties and is used in medicine to improve blood rheology. Separate administration of Longolitine showed moderate activation of fibrinolysis, increasing after discontinuation of the drug by the 12th day, and an increase in anticoagulant and antipolymerization activity by the 6th day. Piyavit's individual use also activated fibrinolysis by day 12 and significantly in-creased the polymerization and anticoagulant properties of blood. When drugs were used together, the plasma anticoagulant activity was slightly potentiat-ed compared with the administration of Longolitine alone. Plasma fibrinolytic activity slightly increased by the 6th day, but significantly and reliably inhibited after drug withdrawal by the 12th day, which is unfavorable for the prevention of thrombosis.

anticoagulant activity
antithrombotic drugs (preparations)

  1. Kieliszek A., Lipinski B. Pathophysiological significance of protein hydrophobic interactions. An emerging hypothesis // Medical hypothesis. 2018. V. 110. № 1. P. 15-22.
  2. Dabbagh E., Negahdaripour M., Berenjcan A., et. al. Nattokinase: production and application. // Applied microbiology and biotechnology. 2014. V. 98. № 22. R. 9199-9206.
  3. Sharkova T.S., Maksimov V.N. Izuchenie fiziologii hischnogo griba Arthrobotrys longa – produtsenta longolitina v svjazi s dlitel'nym hraneniem kul'tury v laboratornyh uslovijah // Mikologija i fitopatologija. 1999. T. 33. № 5. S. 66-71.
  4. Podorol'skaja L.V, Sharkova. T.S, Serebrjakova T.N. i dr. Ekperimental'nyj tromboz na kraevoj vene uha krolika // Bjulleten' eksperimental'noj biologii i meditsiny. 2007. T. 143. № 5. S. 511-515.
  5. Bizulevicius G.A. Where do immunostimulotory effects of oral proteolytic enzymes (« systemic enzyme therapy») come from. Microbal proteolysis as a possible starting point // Med. Hypothesis. 2006. V. 67. № 6. R. 1386-1388.
  6. Sevost'janov V.I., Perova N.V., Dovzhin N.A. i dr. Issledovanie diffuzii lekarstvennogo sredstva pijavit cherez rogovitsu glaza krolika // Eksperimental'naja i klinicheskaja farmakologija. 2005. T. 68. № 1. S. 71-73.
  7. Pavlova I.B., Podorol'skaja L.V., Sharkova T.S. Izuchenie vzaimodejstvija tromboliticheskogo preparata longolitina s nekotorymi ionami i lekarstvennymi sredstvami in vitro // Sovremennye tehnologii v meditsine. 2016. T. 8. № 4. S. 309–312.
  8. Ljapina L.A., Grigor'eva M.E., Obergan T.Ju. i dr. Teoreticheskie i prakticheskie voprosy izuchenija funktsional'nogo sostojanija protivosvertyvajuschej sistemy. M.: OOO «Avansend Soljushn». 2012. 160 s.
  9. Basanova A.V., Baskova I.P., Zavalova L.L. Reguljatory trombotsitarno sosudistogo i plazmennogo zven'ev gemostaza iz krovososuschih // Biohimija. 2002. T. 67. № 1.
  10. S. 167-176.
  11. Podorol'skaja L.V., Sharkova T.S. Effekt neinvazivnyh sposobov vvedenija tromboliticheskogo proteinaznogo kompleksa longolitina // Tromboz, gemostaz, reologija. 2016. T. 67. № 3. S. 334-335.
  12. Podorolskaya L., Sharkova T. Changes of blood fibrinogen concentration, connected to geomagnetical events in healthy rats population // Thrombosis research. 2016. V. 141. Suppl. 1. R. 561.