THE COMPARATIVE ANALYSIS OF THE BIOLOGICALLY ACTIVE SUBSTANCES IN TINCTURA EUCALYPTI AND CHLOROPHYLLIPT MEDICINES

The aim of this article was to carried out the comparative analysis of the main types of the biologically active substances in the tinctura Eucalypti and in the Chlorophyllipt medicine from the different manufacturers by reverse phase high performance liquid chromatography (RP HPLC). Materials and methods. For the studies were used six samples of tinctura Eucalypti and three samples of Chlorophyllipt medicine. The standards as rutin, chlorogenic acid and chlorophyllipt spissum extract from the Eucalyptus globulus Labill. leaves of State Pharmacopoeia of Ukraine have been used in the studies. The analyses of medicines were carried out by chromatograph of Agilent Technologies 1200 Infinity made in USA. The registration of chromatograms was carried out by diode-array detector at following wavelengths: 275, 325, 350, and 660 nm. Results and discussion. Content of chlorogenic acid (from 0.086 to 0.162 mg/ml), rutin (from 0.14 to 0.63 mg/ml), and euglobals (from 1.3 to 23.9 mg/ml equivalent to Pharmacopeia standard of chlorophyllipt spissum extract) were determined in the tincture by means of HPLC analysis. Euglobals (from 9.6 to 39.1 equivalent to Pharmacopeia standard of chlorophyllipt spissum extract) were determined in Chlorophyllipt medicine. In the Chlorophyllipt medicine from the Russian manufacturer was found the larger quantity of euglobals in comparison to medicines of Ukrainian manufacturers. In the tinctures was found correlation between the concentration of euglobals and concentration of rutin. In the tinctures of Ukrainian manufacturers was found that concentration of euglobals was greater than in the Russian manufacturers and has approximately same level of their concentration in Chlorophyllipt medi-cine. Conclusions. In general as a result of carried out of studies we can conclude follows: in the Chlorophyllipt medicine were found two types of eu-globals, one part of them were more hydrophilic and another part were more hydrophobic; in the tinctures were found more hydrophilic types of euglob-als; therefore very perspective the development of package technology of eucalyptus leaves retreatment that gives the possibility firstly extracted the hy-drophobic euglobals, and than obtained the extract with hydrophilic substances.