HYDROLIZABLE GALLO-ELLAGI-TANNINS OF CHAMAENERION ANGUSTIFOLIUM (L.) ARE PROSPECTING CYTOTOXIC SOURCES FOR USE IN ONCOLOGY

DOI: https://doi.org/10.29296/25877313-2019-01-04
Issue: 
1
Year: 
2019

S.A. Sasov Ph.D. (Chem.), Senior Research Scientist, N.N. Blokhin National Medical Research Center of Oncology (Moscow) N.N. Totoeva Leading Laboratory Engineer, N.N. Blokhin National Medical Research Center of Oncology (Moscow) V.N. Tolkachev Leading Research Scientist, N.N. Blokhin National Medical Research Center of Oncology (Moscow) T.A. Alieva Laboratory Assistant, N.N. Blokhin National Medical Research Center of Oncology (Moscow) E.N. Bedrina Research Scientist, N.N. Blokhin National Medical Research Center of Oncology (Moscow) O.N. Tolkachev Dr.Sc. (Chem.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: vilarnii@mail.ru V.A. Misyurin Ph.D. (Biol.), Senior Research Scientist, N.N. Blokhin National Medical Research Center of Oncology (Moscow) L.A. Kesaeva Research Assistant, N.N. Blokhin National Medical Research Center of Oncology (Moscow) Yu.P. Finashutina Research Scientist, N.N. Blokhin National Medical Research Center of Oncology (Moscow) N.A. Lyzhko Research Assistant, N.N. Blokhin National Medical Research Center of Oncology (Moscow) A.V. Misyurin Dr.Sc. (Biol.), N.N. Blokhin National Medical Research Center of Oncology (Moscow) V.Yu. Maslyakov Ph.D. (Geogr.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: maslyakoff@mail.ru N.B. Fadeev Senior Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: nfadeev@mail.ru

There was studied new domestic phytodrug from Chamaenerion angustifolium (L.) as the source for prophylaxis and treatment of oncological dis-eases containing oligomeric hydrolyzing hallo-ellagi-tannins. Isolation and the chemical composition study of antitumor and immune-modulatory activity of dimeric and trimeric tannins from Chamaenerion angustifolium (L.) were carried out using selective extraction and chromatographic separation methods of polyphenols. Flow cytometry was used for evaluation of B cell response after mice immunization. PRAME-expressed cell line K562 was used as sub-strate. Chemical composition of gallo-ellagi-tannins from flowers and shoots were studied. Technologic method of dimeruc gallo-ellagi-tannins chamenerin I production plants was worked out. Possibility of dimeric tannins use to stimulate B cell response was proposed.

Keywords: 
Chamaenerion angustifolium (L.)
oligomeric gallo-ellogi-tannins
hamenerin I
immunization with PRAME protein

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