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A STUDY ON MEMBRANE-STABILIZING AND ANTIOXIDANT ACTIVITY OF THE HERBAL REMEDY IN IN VITRO MODELS

DOI: https://doi.org/10.29296/25877313-2019-01-05
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Issue: 
1
Year: 
2019

S.V. Tishkovets Post-graduate Student, Institute of General and Experimental Biology SB RAS (Ulan-Ude) E-mail: tcb-amur@yandex.ru A.A. Toropova Ph.D. (Biol.), Research Scientist, Institute of General and Experimental Biology SB RAS (Ulan-Ude) A.G. Mondodoev Dr.Sc. (Med.), Senior Research Scientist, Institute of General and Experimental Biology SB RAS (Ulan-Ude) Ya.G. Razuvaeva Dr.Sc. (Biol.), Senior Research Scientist, Institute of General and Experimental Biology SB RAS (Ulan-Ude), Irkutsk State Medical Academy of Postgraduate Education - Branch Campus of the Russian Medical Academy of Continuing Professional Education (Irkutsk) T.V. Kornopoltseva Ph.D. (Pharm.), Research Scientist, Institute of General and Experimental Biology SB RAS (Ulan-Ude)

The membrane-stabilizing, anti-radical and antioxidant activities of a complex herbal remedy preparation in in vitro models were studied. It was shown that the herbal remedy studied inhibits the process of lipid peroxidation, contributing to the stabilization of biological membranes (IC50=12.6 μg/ml), reduces the intensity of osmotic hemolysis (IC50=0.51 μg/ml), which is apparently due to rutin, luteolin and pectin polysaccharide lemnane included in its composition. Сomplex herbal remedy shows pronounced antioxidant and antiradical properties: it prevents oxidation of β-carotene from peroxide degradation (IC50=18.6 μg/ml) and inhibits lipoprotein degradation in metal oxidized oxidation (IC50=75.3 μg/ml); inactivates reactive molecules: DPPH-radical (IC50=30.2 μg/ml), O2•-radical (IC50=32.7 μg/ml), NO (IC50=0.45 mg/ml) and Fe2+ (IC50=3.3 mg/ml). The revealed activity of the studied herbal remedy is caused by a complex of biologically active substances: flavonoids, terpenoids, polysaccharides, carotenoids, phenol carboxylic acids as well as ascorbic and pantothenic acids included in its composition. In this regard, it is promising and relevant to study the pharmacological activity of this herbal remedy, according to the results of which it will be possible to recommend it in the complex therapy of patients with hypothyroidism, for normalization of metabolic processes in the body.
Keywords: 
complex herbal remedy
Juglans regia L. Rich ex Kimth.
Corylus avellana L.
Agrimónia eupatória L.
Bidens tripartita L.
Xanthium strumarium L.
Urtica dioica L. Lemna minor L.
Cichorium intybus L.
Onopordum acanthium L.
membrane-stabilizing activity
antioxidant activity
free radicals

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References: 
  1. Yasenyavskaya A.L. Izuchenie vliyaniya immobilizacionnogo stressa i antioksidantov na gormonal`nuyu aktivnost` shhitovidnoy zhelezy belyh krys na raznyh e`tapah ontogeneza // Vestnik nizhegorodskogo universiteta im. N.I. Lobachevskogo. 2010. T. 2. № 2. S. 689–693.
  2. Urazova O.I., Kravec E.B., Novickiy V.V., Rogaleva A.B., Vasil`eva O.A., Kuznecova V.N., Nedosekova Yu.V. Aktivnost` perekisnogo okisleniya lipido i sistemy glutationa v limfocitah krovi u bol`nyh diffuznym toksicheskim zobom // Byulleten` sibirskoy mediciny. 2008. T. 7. № 4. S. 47–51.
  3. Nasiry D., Khalatbary A.R., Ah-madvand H., Amiri F.T., Akbari E. Protec-tive effects of methanolic extract of Junglans regia L. leaf on streptozotocin-induced diabetic peripheral neuropathy in rats // BMS Complementary and Alterna-tive Medicine. 2017. V. 17. № 1. P. 476–485. doi: 10.1186/s12906-017-1983-x
  4. Dedov I.I., Mel`nichenko G.A., Fadeev V.V. E`ndokrinologiya. M.: GE`OTAR-Media. 2012. 432 s.
  5. Savust`yanenko A.V. Terapiya pervichnogo gipotireoza α-lipoevoy kislotoy. Obzor rezul`tatov issledovaniy // Mizhnarodniy endokrinologichniy zhurnal. 2016. T. 76.
  6. № 4. S. 33–40.
  7. Vladimirov Yu.A. Biologicheskie membrany i nezaprogrammirovannaya gibel` kletki // Sorosovckiy obrazovatel`nyy zhurnal. 2000. T. 6. № 9. S. 2–9.
  8. Korsun V.F. Fitoterapiya. Tradicii rossiyskogo travnichestva. M.: E`ksmo. 2010. 880 s.
  9. Tishkovec S.V., Mondodoev A.G., Razuvaeva Ya.G., Toropova A.A., Cydendambaev P.B., Lobanov A.K., Korsun V.F., Korsun E.V., Shoyzhonova E`.B. Fitokorrekciya narusheniy gormonal`nogo statusa i pokazateley serdechno-sosudistoy sistemy u belyh krys pri e`ksperimental`nom gipotireoze // Materialy III Mezhdunar. nauchno-praktich. konf. Respublika Tyva. 2017. S. 101–104.
  10. Rastitel`nye resursy Rossii: Dikorastushhie cvetkovye rasteniya, ih komponentnyy sostav i biologicheskaya aktivnost`. T. 1. Semeystva Magnoli-aceae – Julandaceae, Ulmaceae, Morace-ae, Cannabaceae, Urticaceae / Otv. red. A.L. Budancev. SPb. 2008. 421 s.
  11. Rastitel`nye resursy Rossii: dikorastushhie cvetkovye rasteniya, ih komponentnyy sostav i biologicheskaya aktivnost`. T. 2. Semeystva Actinidiace-ae-Malvaceae, Euphorbiaceae-Haloragacea / Otv. red. A.L. Budancev. SPb. 2009. 513 s.
  12. Rastitel`nye resursy Rossii: dikorastushhie cvetkovye rasteniya, ih komponentnyy sostav i biologicheskaya aktivnost`. T. 5. Semeystva Asteraceae (Compositae). Ch. 1. Rody Achillea-Doronicum / Otv. red. A.L. Budancev. SPb. 2012. 317 s.
  13. Rastitel`nye resursy Rossii: dikorastushhie cvetkovye rasteniya, ih komponentnyy sostav i biologicheskaya aktivnost`. T. 5. Semeystva Asteraceae (Compositae). Ch. 2. Rody Achillea-Youngia / Otv. red. A.L. Budancev. SPb. 2013. 312 s.
  14. Rastitel`nye resursy Rossii: dikorastushhie cvetkovye rasteniya, ih komponentnyy sostav i biologicheskaya aktivnost`. T. 6. Semeystva Butomaceae – Typhaceae / Otv. red. A.L. Budancev. SPb. 2014. 390 s.
  15. Kovalev I.E., Danilova N.P., Andronati S.A. Vliyanie e`nomelanina na gemoliz e`ritrocitov, vyzyvaemyy svobodnoradikal`nymi reakciyami i drugim faktorami // Farmakologiya i toksikologiya. 1986. № 4. S. 89–91.
  16. Olennikov D.N., Tankhaeva L.M. Lamiaceae carbohydrates. I. Pectinic sub-stances and hemicelluloses from Mentha x piperita // Chemistry of Natural Com-pounds. 2007. V. 43. № 5.
  17. P. 501–507.
  18. Klebanov G.I., Babenkov I.V., Teselkina Yu.O., Komarov O.S., Vladimirov Yu.A. Ocenka antiokislitel`noy aktivnosti plazmy krovi s primeneniem lipoproteidov // Laboratornoe delo. 1988. № 5. S. 59–62
  19. Adesanwo J.K., Makinde O.O., Obafemi C.A. Phytochemical analysis and antioxidant activity of methanol extract and betulinic acid isolated from the roots of Tetracera potatoria // Journal of Phar-macy Research. 2013. V. 6. P. 903–907.
  20. Rahini D., Anuradha R. In vitro an-tioxidant activity of Artabotrys hexapetal-lus // Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2014. V. 5. № 2. P. 396–405.
  21. Olennikov D.N., Zilfikarov I.N., Toropova A.A, Ibragimov T.A. Himicheskiy sostav soka kallizii dushistoy (Sallisia fragrans Wood) i ego antioksidantnaya aktivnost` (in vitro) // Himiya rastitel`nogo syr`ya. 2008. № 4. S. 95–100.
  22. Derffel` K. Statistika v analiticheskoy himii. M.: Mir, 1994. 98 s.
  23. Dayronas Zh.V., Kuleshova S.A., Pshukova I.V. Fitohimicheskoe izuchenie list`ev greckogo oreha kak istochnika antioksidantnogo sredstva // Himiya rastitel`nogo syr`ya. 2010. № 4. S. 95–98.
  24. Santos T.N., Costa G., Ferreira J.P., Liberal J., Francisco V., Paranhos A., Cruz M.T., Castelo-Branco M., Figueiredo V.I., Teresa Batista M. Antioxidant, anti-Inflammatory, and analgesic activities of Agrimonia eupatoria L. Infusion // Evidence-Based Complementary and Alternative Medicine. 2017. V. 2017. Article ID 8309894. 13 pages. https://doi.org/10.1155/2017/8309894.
  25. Bushueva G.R., Strelkova L.B., Kondakova N.V. Issledovanie biologicheskoy aktivnosti durnishnika obyknovennogo travy e`kstrakta suhogo i otdel`nyh frakciy s primeneniem specificheskih fermentativnyh biotest-sistem v usloviyah in vitro // Voprosy biologicheskoy, medicinskoy i farmacevticheskoy himii. 2016. № 6. S. 25–29.
  26. Grineeva O.V., Slivkin A.I., Safonova E.F. Opredelenie gidroskikorichnyh kislot, karotinoidov i hlorofilla v list`yah krapivy dvudomnoy (Urtica dioica L.) // Himiya rastitel`nogo syr`ya. 2015. № 3. S. 105–110.
  27. Adekenov S.M., Danilec M.G., Ivasenko S.A., Nikiforov L.A., Krivoshhekov S.V., Ligacheva A.A., Trofimova E.S., Shestoboev E.Yu., Zhdanov V.V., Belousov M.V. Fenol`nye soedineniya e`tanol`nyh izvlecheniy Lemna minor L., Lemna trisulca L. i Lemna polyrrhiza L. Schleid. i ih immunomoduliruyushhaya aktivnost` // Byulleten` sibirskoy mediciny. 2017. T. 16. № 3. S. 5–15.