PRODUCTION OF IMPLANT PREPARATIONS WITH ANTIMICROBIAL PROPERTIES ON THE BASIS OF STERILE DEMINERALIZED BONE MATRIX

DOI: https://doi.org/10.29296/25877313-2019-03-04
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Issue: 
3
Year: 
2019

Y.Yu. Litvinov Senior Research Scientist, Special Work Department of Research and Educational Center of Biomedical Technologies, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: vilar.litvinov@mail.ru

The results of experimentally tested biotechnological methods within the framework of improved methodology of manufacturing, quality control and safety assessment of bone implants and implant preparations are presented. Materials and methods: improved methodology for obtaining bone implants and implant preparations included the following methods of obtaining implants and their control: biotechnological methods of bone processing, microscopic analysis using light and scanning microscopy, fractographic analysis of bone using precision 3D scanning, elemental analysis using spectroscopy,improved method of sterilization using the ozone method, biomechanical tests for compressive strength and measurement of microhardness, microbiological control after the combined method of sterilization of implants, the method of bone demineralization, method of immobilization of the drug on the basis of demineralized bone matrix and spectrophotometric control of drug release. Conducted verification of the authenticity of the released drug alkaloids of sangvinarin and heleritrine. Results and discussion: the results of experimentally tested methods of obtaining and quality control of bone implants and implant preparations are presented. А biotechnological algorithm algorithm for obtaining bone implants, demineralized implants and implant preparations was developed. Summary:the fundamentals of the technology of production of the bone implant of drugs using the substance of alkaloids sangvinarin and hel-erythrine for the purposes of bioimplantology in the treatment of purulent-necrotic foci in the bone to replace diseased tissue with an implant with strong antimicrobial properties. Developed criteria for quality control cortical bone of the diaphysis of the femur of an ox and obtained from her xenoimplants.

Keywords: 
bone implant
demineralized bone implant
implant preparation
sangviritrin
combined method of sterilization
biomechanical tests
elemental analysis
absorption spectroscopy

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