VOLATILE COMPONENTS OF HOMEOPATHIC MOTHER TINCTURES OF MOSCHUS MOSCHIFERUS

The article presents data on the analysis by GC-MS. of volatile components contained in homeopathic mother tinctures of Moschus moschiferus. Tinctures made by maceration of the crushed raw materials with alcohol 30% (v/v) of three samples dried musk gland prepared according to sup-pliers, winter of 2016 (samples No. 1 and No. 2) and 2017 (sample No. 3) in Altai. The separation and identification were carried out using GC-MS with Varian 450-GC/220-MS equipped with a column Factor Four VF-5ms. In the chromatograph injector at a temperature of 200 °С (split ratio 4), 1 µl sample is injected. Column temperature program: 50 °С - 5 min, heating to 110 °С at a rate of 5ºС/min, 110 °С - 2 min, heating to 240 °С - 25ºС/min, isotherm at 240 °С 15 min. In tinctures 82 components are defined. Samples differ among themselves on composition of the acids, alcohols, ketones, lactones, hydrocarbons, furan derivatives. The similarity consists that the main components of tests are steroids (androgens): 3-hydroxy- (3α,5β)-androstan-17-one (from 13,33 to 33,81% in test), a prasterone acetate (from 1,6 to 5, 39%), 17 - hydroxy - androst-2-en-4-one (from 0,17 to 10,19%), androsterone (from 1,06 to 2,54%), epiandrosterone (from 0,05 to 1,4%), androstenedione (from 0,05 to 0,47%), a trans-dehydroandrosterone, pentafluoropropionate (from 0,64 to 2,50%). Common compounds are an unknown alkaloid or alkaloid-like substance (from 0.28 to 4.27%); %); tetrahydro-5-methyl furfuryl alcohol (0.24 to 0.66%); ethyl esters of fatty acids - palmitic (from 0,3 to 0,7%), 15-methyl-hexadecanoic (from 0.05 to 0.20), stearic (from 0.09 to 0.26); butyl-2-methylhept-3-yl ester phthalic acid (1.8 to 6.56%), hydrocarbons, phenyl-propanedioic or benzeneacetic acid. An important element of similarity is the absence of muskon in the samples. Perhaps this is a feature of the musk of some populations of musk deer populations inhabiting Russia, or due to other factors that require further study in order to establish the active musk components causing its pharmacological efficacy. Therefore, to standardize and develop a quantitative method for inclusion in the pharmacopoeial monograph of the homeopathic mother tincture of Moschus moschiferus, it is preferable to determine the total amount of steroids (androgens).