Нажмите на эту строку чтобы перейти к Новостям сайта "Русский врач"

на сайт
Перейти на сайт журнала "Медицинская сестра"
Перейти на сайт журнала "Фармация"
Перейти на сайт журнала "Молекулярная медицина"
Перейти на сайт журнала "Вопросы биологической, медицинской и фармацевтической химии"
Журнал включен в российские и международные библиотечные и реферативные базы данных

ВАК (Россия)
РИНЦ (Россия)
Эко-Вектор (Россия)


DOI: https://doi.org/10.29296/25877313-2019-03-05
Download full text PDF

Ya.F. Kopytko Ph.D. (Pharm.), Всероссийский научно-исследовательский институт лекарственных и ароматических растений (Москва) E-mail: yanina@kopytko.ru N.S. Tsibulko Ph.D. (Pharm.), Всероссийский научно-исследовательский институт лекарственных и ароматических растений (Москва)

The article presents data on the analysis by GC-MS. of volatile components contained in homeopathic mother tinctures of Moschus moschiferus. Tinctures made by maceration of the crushed raw materials with alcohol 30% (v/v) of three samples dried musk gland prepared according to sup-pliers, winter of 2016 (samples No. 1 and No. 2) and 2017 (sample No. 3) in Altai. The separation and identification were carried out using GC-MS with Varian 450-GC/220-MS equipped with a column Factor Four VF-5ms. In the chromatograph injector at a temperature of 200 °С (split ratio 4), 1 µl sample is injected. Column temperature program: 50 °С - 5 min, heating to 110 °С at a rate of 5ºС/min, 110 °С - 2 min, heating to 240 °С - 25ºС/min, isotherm at 240 °С 15 min. In tinctures 82 components are defined. Samples differ among themselves on composition of the acids, alcohols, ketones, lactones, hydrocarbons, furan derivatives. The similarity consists that the main components of tests are steroids (androgens): 3-hydroxy- (3α,5β)-androstan-17-one (from 13,33 to 33,81% in test), a prasterone acetate (from 1,6 to 5, 39%), 17 - hydroxy - androst-2-en-4-one (from 0,17 to 10,19%), androsterone (from 1,06 to 2,54%), epiandrosterone (from 0,05 to 1,4%), androstenedione (from 0,05 to 0,47%), a trans-dehydroandrosterone, pentafluoropropionate (from 0,64 to 2,50%). Common compounds are an unknown alkaloid or alkaloid-like substance (from 0.28 to 4.27%); %); tetrahydro-5-methyl furfuryl alcohol (0.24 to 0.66%); ethyl esters of fatty acids - palmitic (from 0,3 to 0,7%), 15-methyl-hexadecanoic (from 0.05 to 0.20), stearic (from 0.09 to 0.26); butyl-2-methylhept-3-yl ester phthalic acid (1.8 to 6.56%), hydrocarbons, phenyl-propanedioic or benzeneacetic acid. An important element of similarity is the absence of muskon in the samples. Perhaps this is a feature of the musk of some populations of musk deer populations inhabiting Russia, or due to other factors that require further study in order to establish the active musk components causing its pharmacological efficacy. Therefore, to standardize and develop a quantitative method for inclusion in the pharmacopoeial monograph of the homeopathic mother tincture of Moschus moschiferus, it is preferable to determine the total amount of steroids (androgens).

Moschus moschiferus
homeopathic tincture

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.

  1. 1. Grech-Baran Marta, Sykłowska-Baranek Katarzyna, Pietrosiuk Agnieszka. Biotechnological approaches to enhance salidroside, rosin and its derivatives production in selected Rhodiola spp. in vitro cultures // Phytochem Rev. 2015. № 14. S. 657–674.
  2. 2. Lupanova I.A., Strelkova L.B., Savina T.A., Ferubko E.V. Study of biological activity of the Rhodiola rosea L. biomass extract using specific enzymatic test systems in vitro // Scientific-practical journal «Issues of quality assurance of medicines». 2017. № 3 (17). P. 9–15.
  3. 3. Savina T.A., Myasnikova S.B., Balakina M.V. Optim ization of conditions of cultivation for suspension culture of the Rhodiola rosea L. // Questions of biological, medical and pharmaceutical chemistry. 2016. №. 9. C. 13–18.
  4. 4. Savina T.A., Myasnikova S.B., Savin P.S. The influence of the composition of the nutrient medium on the growth and biosynthetic parameters of suspension culture of Rhodiola rosea (Rhodiola rosea L.) // Questions of biological medical and pharmaceutical chemistry. M. 2018. № 8. P. 9–12.
  5. 5. Lezhnev E.I., Popova I.I., Lavrovskaya V.P. Measurement error correction of Clark oxygen electrode Autoconsumption //Scientific instrument engineering. M. V. 18. №. 1. 2008. Р. 76–81.
  6. 6. Vasil'ev N.N., Ambrosov V.A., Skladnev A.A. Modelirovanie processov mikrobiologicheskogo sinteza [Modelling of microbiological synthesis processes]. Moscow, Lesn. prom. Publ. 1975. 344 p.
  7. 7. Tsybulko N., Savina T. A. Method for the quantitative determination of phenylpropanoids in cell culture Rhodiola rosea // From plant to drug: tradition and modernity: collection of scientific works. Tr. GNU VILAR. M. 2014. Р. 48–52.
  8. Li D., Chen B., Zhang L., et al. The musk chemical composition and microbiota of Chinese forest musk deer males // Sci Rep. 2016; 6:18975.
  9. Burdock G.A. Fenaroli’s Handbook of Flavor Ingredients. Fourth Edition CRC Press. 2001. 1864 pp.
  10. Sokolov V.E., Kagan M.Z., Vasilieva V.S., et al. Musk deer (Moschus moschiferus): reinvestigation of main lipid components from preputial gland secretion // J Chem Ecol. 1987; 13:71-83.
  11. Pharmacopoeia of the People’s Republic of China (English Edition). V. 1. Chinese Pharmacopoeia Comission. People’s Medical Publishing House. 2005. R. 168-169.