DEVELOPMENT OF THE METHOD OF QUANTITATIVE ANALYSIS OF PENOLIC COMPOUNDS IN HERB MOLDAVIAN DRACONHEAD (DRACOCEPHALUM MOLDAVICA L.)

DOI: https://doi.org/10.29296/25877313-2019-04-02
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Issue: 
4
Year: 
2019

E.V. Zvezdina Post-graduate Student, Senior Researcher, Phytochemistry Department, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) O.P. Sheichenko Ph.D. (Chem.), Head of Phytochemistry Department, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: vilarnii.sheychenko@mail.ru

The purpose of the work is selection of optimal conditions for methods of authenticating and quantifying the amount of phenolic compounds in the herb of the moldavian draconhead (Dracocephalum moldavica L.) Material and methods. The objects of the study were samples of the dried above-ground part of the Dracocephalum moldavica L., harvested in the Botanical Garden, the Middle Volga and North Caucasus branches of All-Russian Scientific Research Institute of Medicinal and Aromatic Plants from 2016-2018. To characterize authenticity of this raw material, a sensitive reaction with 3% solution of iron chloride was used for determine action of phenolic compounds. To determine rosmarinic acid, thin layer chromatography on sorbent (TLC) was used. Sorbfil PTSH-PA plates measuring 10 × 15 were used as the stationary phase. Chromatography was carried out in an ascending fashion using an ethyl acetate – acetic acid – water solvent system (40: 5: 5). In experiments were used standard sample (CO) of rosmarinic acid (Sigma Aldrich, cat. No. 536954-5G). UV absorption spectra were recorded on spec-trophotometers UV-1800 (Shimadsu), Cary 100 Scan (Varian). Results and conclusions. Dracocephalum moldavica L. is prospective source for production of new medicinal remedies. Results of the methods of their products are worked out in order to worked out of their identity using novel quality tests and TLC. There was found condition and there was worked out methods of qualitative determination of the total phenolic compounds calculated on rosmarinic acids in Dracocephalum moldavica L. herb using direct spectrophotometric method and their validation was carried out according to the following indicators: specificity, linearity, correctness, intralaboratory precision: convergence and reproducibility.

Keywords: 
Dracocephalum moldavica L. herb
phenolic compounds
rosmarinic acid
spectrophotometry
validation

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