MODERN METHOD OF ELEMENTAL ANALYSIS OF MINERALIZED TISSUE USING ENERGY DISPERSIVE X-RAY SPECTROSCOPY

DOI: https://doi.org/10.29296/25877313-2019-04-03
Issue: 
4
Year: 
2019

Y.Yu. Litvinov Senior Research Scientist, Special Work Department of Research and Educational Center of Biomedical Technologies, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: vilar.litvinov@mail.ru

The results of experimentally tested modern method of elemental analysis of mineralized tissue using energy dispersive x-ray spectroscopy are presented. Material and methods: it have been studied 4 groups of bone samples of 5 pieces each: 1) native bone samples (control group); 2) bone samples, after incubation in hydrochloric acid 0.8 n for 24 hours; 3) bone samples, after incubation in hydrochloric acid 0.8 n for 48 hours; 4) bone samples, after incubation in hydrochloric acid 0.8 n for 72 hours. Evaluation and control of the degree of demineralization of bone implants was performed using scanning electron microscope Phenom XL (Phenom-World, the Netherlands), equipped with a detector for integrated energy dispersive x-ray spectroscopy. Results and discussion: the control showed that after 72 hours of incubation in hydrochloric acid 0.8 n, the presence of - CA, Na, Mg is not de-termined in the bone matrix according to x-ray spectroscopy. The relative percentage of P has decreased by 45 times. The relative percentage of N has increased by 2.5 times and C has increased by 4.5 times. Summary: experimentally tested method of elemental analysis using energy dispersive x-ray spectroscopy can be used to control the degree of demineralization of bone tissue in the preparation of bone implants for bioimplantology.

Keywords: 
x-ray spectroscopy
bioimplantology
osteogenesis
demineralization

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