IN VIVO INVESTGATION OF ALLERGENIC AND IMMUNOTOXICITY PROPERTIES OF REDLEAVES EXTRACT ISOLATED FROM VITIS VINIFERA L

DOI: https://doi.org/10.29296/25877313-2019-04-08
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Issue: 
4
Year: 
2019

V.V. Bortnikova Ph.D. (Biol.), Leading Research Scientist, Department of Toxicology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: bortnikova.v@yandex.ru L.V. Krepkova Ph.D. (Biol.), Head of Toxicology Department, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) Е-mail: krepkowa2011@yandex.ru А.N. Babenko Ph.D. (Рharm.), Ph.D. (Biol.), Leading Research Scientist, Department of Toxicology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) Е-mail: alexandra.mogileva@gmail.com M.V. Вorovkova Senior Research Scientist, Toxicology Department, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) Е-mail: borovkova_65@mail.ru O.S. Kuzina Senior Research Scientist, Toxicology Department, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) Е-mail: oskt@list.ru

Рurpose: complex assessment of the influence of grape culture (Vitis vinifera L.) leaves of red extract of dry on the immune system of experi-mental animals at the stage of preclinical studies. Methods: experiments were performed on albinos males guinea pigs (body weight 350-400 g), CBA and BALB/c males mice (body weight 18-20 g). Allergenicity was studied in tests of general systemic anaphylaxis (anaphylactic shock) and active skin anaphylaxis, as well as in delayed hypersensitivity reaction(HRT). Humoral immunity was assessed by the test of the generation of antibody-forming cells in the spleen by the method of local hemolysis in the modification Cunningham and the titer of hemagglutinins in the reaction of hemagglutination in the serum raised in microtitration Takachi. The state of T-cell immunity was investigated by induction test in the reaction HRT of CBA mice. Results: it was shown that the studied extract in the tested doses of 10 and 100 mg/kg and sensitization schemes did not cause a reaction of general anaphylaxis (anaphylactic shock) and active skin anaphylaxis carried out on albino guinea pigs. Grape leaf red extract dry, introduced with PAF, did not potentiate inflammation in the reaction HRT of BALB/c mice in the study of immunotoxic properties, it was found that the studied extract showed a stimulating dose-dependent effect on the primary humoral immune response and had no effect on the effectors of the cellular immunity of experimental animals. Conclusions: studies have shown that the grape leaf red extract dry did not have a sensitizing effect. The stimulating effect of the studied extract on T - dependent immune response of CBA mice was revealed.

Keywords: 
Vitis vinifera
grape red leaf extract dry
allergenicity
immunotoxicity

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