A NOVEL METHOD FOR EXTRACTION OF ESSENTIAL OIL COMPOUNDS FROM FOENICULUM VULGARE MILL. FRUITS

DOI: https://doi.org/10.29296/25877313-2019-05-01
Download full text PDF
Issue: 
5
Year: 
2019

N.N. Boyko Ph.D. (Pharm.), Associate Professor, Department of Pharmaceutical Technology, Belgorod National Research E-mail: boykoniknik@gmail.com; ORCID: 0000-0001-9222-2935 D.I. Pisarev Dr.Sc. (Pharm.), Professor, Department of Chemistry, Belgorod National Research E-mail: pisarev@bsu.edu.ru; ORCID: 0000-0002-2996-7712 E.T. Zhilyakova Dr.Sc. (Pharm.), Professor, Head of Department of Pharmaceutical Technology, Belgorod National Research E-mail: ezhilyakova@bsu.edu.ru A.Yu. Maljutina Ph.D. (Pharm.), Associate Professor, Department of Chemistry, Belgorod National Research E-mail: malyutina_a@bsu.edu.ru O.O. Novikov Dr.Sc. (Pharm.), Professor, Head of Test Department «Drugs quality control center» of the Shared Research and Education Center, Рeoples’ Friendship University of Russia (Moscow) E-mail: novikov@bsu.edu.ru; ORCID: 0000-0003-3145-6783

The aim of the work was to prove experimentally the possibility of extraction of main essential oil components from the Foeniculum vulgare Mill. fruits by perfluoro organic solvents Novec 1230 and Novec 7100. Materials and methods. For study purposes, we used Foeniculum vulgare Mill. fruits with particle fraction of 0.1–0.5 mm. Quantitative and qualitative analyses of extracts were carried out by GC-MS and RP-HPLC methods. Two methods of extraction were used: simple maceration, plant raw material / solvent ratio 1:10 w/v, temperature 24±1 °С, maceration time 24 hours; circulation method: plant raw material / solvent ratio 1:5 w/v, solvent circulation time from 1 to 5 hours. Results and discussion. During maceration, the highest yield of anethole was observed in n-hexane, 96±4%, and in solvent Novec 7100, 94±4%, whereas in solvent Novec 1230, the yield of anethole was three times less, 28±2%. Solvents Novec 7100/1230 extracted identical compounds, and trans-anethole was the dominant one among them. The yield of anethole within two hours of solvent circulation was up to 82±4% for Novec 7100, and 89±4% for Novec 1230. Conclusions. The possibility of extraction of main components of essential oil from the plant raw material with perfluoro organic solvents Novec 1230/7100 by the example of Foeniculum vulgare Mill. fruits was experimentally tested. It was determined that the level of solvent molecule fluorination influenced its extraction properties with respect to anethole and fatty oil.

Keywords: 
RP-HPLC
GC-MS
essential oil
anethole
Foeniculum vulgare Mill. fruits
Novec 1230
Novec 7100
n-hexane

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.

References: 
  1. Sidorov I.I., Turisheva N.A., Faleeva L.P., Yasnjukevich E.I. Technology of natural essential oils and synthetic flavor substances / Moscow: Light and food industries, 1984; 368 p. (In Russ.).
  2. Hammouda F.M., Saleh M.A., Abdel-Azim N.S., Shams K.A., Ismail S.I., Shahat A.A., Saleh I.A. Evaluation of the essential oil of Foeniculum vulgare Mill (Fennel) fruits extracted by three different extraction methods by GC/MS // Afr J Tradit Complement Altern Med. 2013; 11(2):277-279.
  3. Dowdle P.A., Merseyside S.S.H., Corr S., Cheshire A., Harris H., Cheshire W. Patent US 2004/0105899 A. Solvent extraction process; Pub. Date: Jun 3, 2004.
  4. Mukhopadhyay M. Natural extracts using supercritical carbon dioxide. 1st ed. / Boca Raton: CRC Press, 2000; 360 p.
  5. Santos D.T., Meireles M.A.A. Extraction of Volatile Oils by Supercritical Fluid Extraction: Patent Survey // Recent Patents on Engineering. 2011; 5(1): 17-22.
  6. Technology and standardization of drugs: Collection of research papers. Ed. By V.P. Georgievskiy and F.A. Konev / Kharkov: LLC “RIREG”, 2000; 784 p. (In Russ.).
  7. Lemaire B., Mompon B., Surbled I., Surbled M. Patent EP 1307533B1. Method for fractionating essential oils using at least fluorinated solvent. Priority date 2000-06-22.
  8. LeMaire B., Surbled M., Zwegers J., Mompon B. Patent US 6673952 B2. Method for extracting and fractionating fats with solvent, using at least a hydrofluroether. Pub. Date: Jan 6, 2004.
  9. Dournel P., Surbled M., Mompon B. Patent US 2006/0182689 A1. Process for separating out at least one organic compound. Pub. Date: Aug 17, 2006.
  10. Industrial solvents handbook. Edited by Ernest W. Flick. 5th ed. / Westwood, New Jersey: Noyes data corporation, 1998; 994 p.
  11. Temperature-controlled liquids 3 M. https://www.3mrussia.ru. (In Russ.).
  12. Barabanov V.G., Blinova O.V., Zotikov V.S., Lizgunov S.A., Orlov A.P., Orlov G.D., Trukshin I.G., Tcelikov V.N. Alternative ozone safe propellants, freons, foaming agent, solvents and fire suppressants / Moscow: 2002; 221 p. (In Russ.).
  13. New in the technology of fluorinated compounds: Ed. By N.I. Isikava / Moscow: Mir, 1984. 592 p. (In Russ.).
  14. Popova N.V., Litvinenko V.I., Kucanjan A.S. Medicinal Plants of the World: Encyclopedic Handbook. Kharkov: Disa Plus; 2016; 540 p. (In Russ.).
  15. Zhilyakova E.T., Novikov O.O., Pisarev D.I., Malyutina A.Y., Boyko N.N. Studying the polyphenolic structure of Laurus Nobilis L. leaves // Indo American Journal of Pharmaceutical Sciences. 2017;4(09):3066-3074.
  16. European Pharmacopoeia. 8th ed. Strasbourg: European Directorate for the Quality of Medicines & HealthCare; 2014.
  17. Zorca M., Gainar I., Bala D. Influence of the process parameters on supercritical СО2 extraction of fennel essential oil // Analele Universitatii Bucuresti: Chimie. XV (serie noua). 2006; (I):107-111.
  18. Kas’yanov G.I., Pehov A.V., Taran A.A. Natural food flavors – CO2 extracts / Moskow: Food industries, 1978; 176 p. (In Russ.).
  19. Girova T., Gochev V., Stoiliva I., Dobreva K., Nenov N., Stanchev V., Stoyanova A. Low temperature extracton of essential oil bearing plants by liquificate gases: Fruits from sweet fennel (Foeniculum officinale Mill.) / Greece: International Scientific Conference eRA-6, 2001; 1-6.