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CHEMICAL-TOXICOLOGICAL DETERMINATION OF FELODIPINE

DOI: https://doi.org/10.29296/25877313-2019-06-04
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Issue: 
6
Year: 
2019

L.L. Kvachakhiya Ph.D. (Pharm.), Associate Professor of the Department Pharmacology, Kursk State Medical University E-mail: Lekso82@yandex.ru V.K. Shormanov Dr.Sc. (Phsrm.), Professor of Department of Pharmaceutical, toxicological and analytical Chemistry, Kursk State Medical University E-mail: R-WLADIMIR@yandex.ru D.S. Sukhomlinov Student, Medical Faculty, Kursk State Medical University E-mail: R-WLADIMIR@yandex.ru

Aim purpose. The search for optimal conditions for isolating felodipine, its purification and the development of methods for determining felodi-pine in biological material. Methods. The object of study is felodipine (3-ethyl-5-methyl-4- (2,3-dichlorophenyl) -2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate). Con-ducted research on the comparative isolation of felodipine from the biomaterial with fluids of organic nature, water and aqueous solutions of various reactions. To perform the experiments, model mixtures of felodipine and crushed liver tissue (particle size 0.2-0.5 cm) with a content of 0.05% of the substance were prepared. Acetone has been proposed as an insulating agent for the recovery of felodipine from biological fluids. For identification and quantification of felodipine in extracts from biological material have been proposed methods of thin layer chromatography (TLC), spectrophotometry, and chromatography – mass spectrometry (GC – MS) Results. It has been established that optimal conditions for the extraction of felodipine from biological material are achieved already with dou-ble infusion of a biological object with an insulating agent, if the mass ratio of the insulating liquid and the biomaterial at each stage of infusion is at least 2 : 1, and the duration of infusion is at least 30 minutes. Optimal purification conditions for felodipine were achieved in the macrocolumn of the Silaborus S-18 sorbent 30 µm in height 150 mm and 10 mm in diameter with elution of the substance with acetonitrile-water polar eluant (7 : 3). When the content of felodipine in the amount of 25 mg in 25 g of biological material, the developed method allows to determine 83.91-85.55% in the liver, 83.82-85.63% in the putrefactive liver of this substance. The openable minimum by the method (100 g of a biological object) is: 0.150 mg of the sub-stance in the liver, 0.220 mg in the rotten liver. Conclusion. Thus, the technique can be applied in the practice of chemical-toxicological analysis to study the tissues of fresh and putrefactive corpse organs for the presence of felodipine in them.

Keywords: 
felodipine
biological material
identification and definition

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