QUANTITATIVE DETERMINATION OF GLYCEROL AND POTASSIUM ACETATE IN AQUEOUS SOLUTION BY THE METHOD OF 1H NUCLEAR MAGNETIC RESONANCE

DOI: https://doi.org/10.29296/25877313-2019-08-02
Issue: 
8
Year: 
2019

V.I. Sheychenko Ph.D. (Phys.-Math.), Leading Research Scientist, All-Russia Scientific Research Institute of Medical and Aromatic Plants (Moscow) E-mail: vladimir.sheychenko@gmail.com Yu.V. Abramov Ph.D. (Biol.), Leading Research Scientist, All-Russia Scientific Research Institute of Medical and Aromatic Plants (Moscow) E-mail: abramov.vilar@yandex.ru G.I. Blinova Senior Research Scientist, All-Russia Scientific Research Institute of Medical and Aromatic Plants (Moscow)

Methodical approaches to the application of 1H NMR spectroscopy for the simultaneous quantitative determination of potassium acetate and glycerol in highly concentrated aqueous solutions. The results obtained can be used in the manufacture and analysis of chemical composition of pre-servative solution used in preservation of biological substances in research institutions and museums biological focus. The 1H NMR spectrum was obtained with the following parameters: response time - 5 seconds; RF pulse length - 3.3 µs (10 degrees); relaxation delay - 10 seconds; the number of accumulations is 16. Then, the integral intensity of the signals is determined in the 1H NMR spectrum, after which the obtained values of the integrals in the ranges of chemical shifts 1.8 - 2.0; 3.4 - 3.8 and 4.6 - 4.9 ppm calculate the molar and mass percentage of each component. The high accuracy of the quantitative determination of the components of the preservative solution and the high economic efficiency compared with the method of potentiometric titration were determined

Keywords: 
1H NMR
preservative solution
potassium acetate
glycerin
quantitative determination
highly concentrated solution

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