THE CURRENT STATE AND PROSPECTS OF USING PROTEOMICS METHODS IN CHEMICAL-TOXICOLOGICAL ANALYSIS (REVIEW)

DOI: https://doi.org/10.29296/25877313-2019-09-04
Issue: 
9
Year: 
2019

V.A. Kutyakov Ph.D. (Biol.), Associate Professor, Department of Biological Chemistry with a Course of Medical, Pharmaceutical and Toxicological Chemistry, Krasnoyarsk State Medical University named after professor V.F. Voyno-Yasenetsky, Ministry of Health of the Russian Federation (Krasnoyarsk) E-mail: victor-koutjakov@yandex.ru E.V. Kharitonova Ph.D. (Pharm.), Senior Lecturer, Department of Biological Chemistry with a Course of Medical, Pharmaceutical and Toxicological Chemistry, Krasnoyarsk State Medical University named after professor V.F. Voyno-Yasenetsky, Ministry of Health of the Russian Federation (Krasnoyarsk) E-mail: ekaterinav1201@gmail.com R.Ya. Olovyannikova Ph.D. (Biol.), Associate Professor, Department of Biological Chemistry with a Course of Medical, Pharmaceutical and Toxicological Chemistry, Krasnoyarsk State Medical University named after professor V.F. Voyno-Yasenetsky, Ministry of Health of the Russian Federation (Krasnoyarsk) E-mail: Olovyannikova2010@yandex.ru A.B. Salmina Dr.Sc. (Med.), Professor, Head of the Department of Biological Chemistry with a Course of Medical, Pharmaceutical and Toxicological Chemistry, Krasnoyarsk State Medical University named after professor V.F. Voyno-Yasenetsky, Ministry of Health of the Russian Federation (Krasnoyarsk) E-mail: allasalmina@mail.ru

Purpose. The authors analyzed the current state and the possibility of using proteomic methods during chemical-toxicological studies. The factors that impede the detection, identification and determination of toxicants in complex biological matrices are indicated. At the same time, the peculiarities of modern expert practice, which determine the introduction of high-tech techniques that differ from those currently used, are noted. The possibility of using dysregulation of gene expression as an alternative method of identifying substances that are harmful to health is con-sidered. As a diagnostic method for diseases of chemical etiology (including substance abuse), it is possible to apply the methods of proteomics. The article provides a comparative description of sample preparation methods, analysis, their advantages and possible disadvantages. The conclusion is made about the need to create biobanks of objects that can serve as benchmarks for comparison in subsequent studies. Emphasis is placed on the need to improve computational analysis tools for processing toxicoproteome data, the creation of mathematical models of poisoning, along with the development of analytical methods. Taking into account the peculiarities of the chemical-toxicological analysis, the authors proposed a scheme (methodology) of a proteomic study of a limited list of objects (blood, urine). Conclusion. Based on the analysis of literature data, the authors of the article propose a new promising tool for carrying out chemical-toxicological studies, which has undoubted advantages - personalized analytical toxicology.

Keywords: 
biomarkers
proteomics
toxicoproteomics
personalized analytical toxicology
chemical-toxicological analysis

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