THE USE OF MEDICINAL PLANT RAW MATERIAL WASTE FOR THE IOTECHNOLOGICAL OBTAINING OF HYDROLYTIC ENZYMES

DOI: https://doi.org/10.29296/25877313-2019-09-06
Issue: 
9
Year: 
2019

Z.K. Nikitina Dr.Sc. (Biol.), Professor, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: nikitinaz@yandex.ru I.K. Gordonova Ph.D. (Biol.), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: gordonova777@yandex.ru

Currently, the drugs based on medicinal plant materials are widely used for the treatment of many chronic diseases. Modern plant-based drugs, as a rule, combine high efficiency, relative safety and wide therapeutic action. When you receive many galenic and neogalenic herbal drugs in most cases, plant raw materials wastes are generate. They usually contain different biopolymers, including cellulose, which can induce under certain condi-tions the hydrolase synthesis. The study is devoted to the solution of the actual problem connected with the use of plant raw materials wastes for hy-drolytic enzymes biotechnological production. As an example of plant material waste were used two samples of Tanacetum vulgare flowers waste. Previously the authors have shown that micromycetes from the collection of GNU VILAR have the ability to hydrolyze some unsoluble proteins and var-ious types of cellulose. In this regard, the attempt to use these biological objects not only for waste conversion, but also to cellulases obtaining was held. It was shown that all the investigated filamentous fungi grew well on media with replacement of sucrose to the Tanacetum vulgare flowers waste. Micromycetes formed lysis zones in surface cultivation and synthesized cellulases in deep cultivation. Thus, approaches to the use of medici-nal plant materials waste for the cellulases production have been developed.

Keywords: 
micromycetes
bacteria
cellulose
plant raw material

References: 
  1. Lekarstvennye rasteniya gosudarstvennoj farmakopei. Farmakognoziya / Pod red. I.A. Samylinoj, V.A. Severceva. M.: AMNI. 1999. 534 s.
  2. Chikov P.S. Lekarstvennye rasteniya. M.: Medicina. 2002. 490 s.
  3. Fitoterapiya s osnovami klinicheskoj farmakologii / Pod red. V.G. Kukesa. M.: Medicina. 1999. 192 s.
  4. Lesiovskaya E.E., Pastushenkov L.V. Farmakoterapiya s osnovami fitoterapii: Ucheb. posobie. M.: GEOTAR-MED. 2003. 592 s.
  5. Medethanov F.A., Ovsyannikov A.P., Hajrullin D.D., Mullakaeva L.A. Tekhnologiya izgotovleniya lekarstvennyh form: Ucheb. posobie. Kazan': FGBOU VO Kaz .GAVM im. N.E. Baumana. 2016. 123 s.
  6. Nikitina Z.K., Gordonova I.K. Ispol'zovanie nativnyh i modificirovannyh belkov kozhi pri poiske producentov proteinaz. // Sbornik nauch. trudov Mezhdunar. konf. «Biologicheskie osobennosti lekarstvennyh i aromaticheskih rastenij i ih rol' v medicine», posvyashchennoj 85-letiyu VILAR 2325 iyunya 2016. 401-5
  7. Nikitina Z.K., Gordonova I.K. Razrabotka metodicheskih podhodov dlya poiska producentov cellyulaz // Voprosy biol., medicinskoj i farmacevticheskoj himii. 2018. № 3. S. 2731. DOI: 29296/25877313-2018-03-05.
  8. Gordonova I.K., Nikitina Z.K., Zon Hy Chol. Sravnitel'naya ocenka proteoliticheskoj aktivnosti bakterij i mikromicetov // Voprosy biologicheskoj, medicinskoj i far-macevticheskoj himii. 2017. № 9. S. 1824.
  9. Gryaznov M.Yu. Izuchenie biologicheskih osobennostej pizhmy obyknovennoj (Tanasetum vulgare) v nechernozemnoj zone Rossii: Avtoref. diss. … kand. biol. nauk. M. 2013. 24 s.
  10. Yakovleva M.B., Nikitina Z.K. Skrining-metody v biotekhnologii (Obzor). Chast' 1. Poisk mikroorganizmov-producentov fermentov // Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2016. № 4. S. 2332.
  11. Sinicyn A.P., Gusakov A.V., Chernoglazov V.M. Biokonversiya ligninocellyuloznyh materialov. M.: Izd-vo MGU. 1995. 224 s.