SCREENING OF PODOPHYLLOTOXIN IN PODOPHYLLUM PELTATUM CELL CULTURE BY THIN-LAYER CHROMATOGRAPHY WITH DIFFERENT EXTRACTION METHODS

DOI: https://doi.org/10.29296/25877313-2020-07-05
Issue: 
7
Year: 
2020

M.P. Kitaeva Post-graduate Student, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: kimape@mail.ru T.A. Savina Ph.D. (Biol.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) T.A. Fedotcheva Ph.D. (Med.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow), Scientific Research Laboratory of Molecular Pharmacology of the Russian National Research Medical University named after N.I. Pirogov (Moscow)

Background. Podophyllum peltatum L. in modern medicine is used as a source of podophyllin (a mixture of resinous substances and lignans) and the cytostatic compound of podophyllotoxin, the main active component of podophyllin. Podophyllin is an effective laxative, has a choleretic effect, is used to enhance the biliary function of the liver. Podophyllotoxinis used to treat viral diseases of the skin and mucous membranes. It is used to remove genital warts, for the treatment of papilloma of the larynx and bladder of scaly lichen and neurodermatitis. The purpose of the research was to study of the influence of methods for extracting the cell culture of P. peltatum on the chemical composition of the extract (podophyllin resin). Material and methods. The object of the study was a suspension cell culture of Podophyllum peltatum L., obtained from the fetus, kidney, and root of a plant located in the collection of All Russian Scientific Research Institute of Medicinal and Aromatic Plants. Screening for the presence of biologically active substances in the podophyllin resin was performed by TLC on plates with a Silufol UV 254 layer of silica gel. Results. Nine variants of extractantswere testedwhen optimizing the extraction conditions. All studied extractants provided the extraction of podophyl-lin resin from the cell culture of P. peltatum. Chloroform and ethyl acetate were the best extractants for the isolation of podophyllin resin with a higher content of podophyllotoxin from P. peltatum cell culture. According to TLC, it is seen that the obtained samples of podophyllin resin contained a large number of compounds. In UV-254 nm, in all cases, adsorption zones corresponding to the position and color of podophyllotoxin were detected. Of the three analyzed cell lines, the highest content of podophyllotoxinwas detected in the cell culture isolated from the plant fruit.

Keywords: 
podophyllinum
podophyllotoxin
Podophyllum peltatum
TLC

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