Нажмите на эту строку чтобы перейти к Новостям сайта "Русский врач"

Перейти
на сайт
журнала
"Врач"
Перейти на сайт журнала "Медицинская сестра"
Перейти на сайт журнала "Фармация"
Перейти на сайт журнала "Молекулярная медицина"
Перейти на сайт журнала "Вопросы биологической, медицинской и фармацевтической химии"
Журнал включен в российские и международные библиотечные и реферативные базы данных

ВАК (Россия)
РИНЦ (Россия)
Эко-Вектор (Россия)

A COMPARATIVE ANALYSIS OF THE CONTENT OF SUGARS AND FLAVONOIDS IN THE HAIRY ROOTS OF SUNFLOWER HELIANTUS ANNUUS L

DOI: https://doi.org/10.29296/25877313-2020-09-02
Download full text PDF
Issue: 
9
Year: 
2020

A.B. Yakupova Ph.D. (Biol.), Bashkir State University (Ufa) E-mail: alfiram@yandex.ru K.G. Musin Junior Research Scientist, Laboratory of Plant Genomics, Institute of Biochemistry and Genetics of Ufa Federal Research Centre of RAS (Ufa) E-mail:khalit.musin@yandex.ru B.R. Kuluev Dr.Sc. (Biol.), Head of the Laboratory of Plant Genomics, Laboratory of Plant Genomics, Institute of Biochemistry and Genetics of Ufa Federal Research Centre of RAS (Ufa) E-mail: kuluev@bk.ru

Relevance. Sunflower Heliantus annuus L. roots are used in the treatment of urolithiasis and cholelithiasis. Harvesting of sunflower roots for medical purposes is fraught with a number of problems, of which the impossibility of year-round cultivation, the absence of devices and machines for collect-ing roots, and contamination of roots with pesticides are most relevant. In this regard, it is of great interest to obtain hairy roots of sunflower that can grow on hormone-free nutrient media in isolated cultures. The aim of our work was to create hairy roots of sunflower using strains A4 and 15834 of Agrobacterium rhizogenes, evaluate their growth parameters when grown on solid media, and analyze their content of water-soluble sugars and flavonoids. Material and methods. The study revealed greater efficiency of strain A4 than strain 15834 for Agrobacterium-mediated transformation of sunflower cotyledon explants. The content of sugars and flavonoids was measured spectrophotometrically using alcohol extracts. Results. The content of water-soluble sugars in the hairy root culture obtained using strain 15834 of A. rhizogenes was more than 2 times higher than that in the hairy roots obtained using strain A4 of A. rhizogenes. Naturally grown sunflower roots contained fewer water-soluble sugars than hairy roots. The total content of flavonoids in sunflower hairy root cultures obtained using strains A4 and 15834 of A. rhizogenes did not differ significantly, but on average it was 2.7 times higher than in native sunflower roots. Conclusion. The hairy roots of sunflower were obtained by agrobacterial transformation of cotyledon explants in vitro. The resulting hairy roots are characterized by unrestricted growth on hormone-free media and a higher water-soluble sugar content than native plant roots.

Keywords: 
Heliantus annuus
sunflower
hairy roots
Agrobacterium rhizogenes
water-soluble sugars
flavonoids

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.

References: 
  1. Gavrilova V.A., Anisimova I.N. Genetika kul’turnyh rastenij. Podsolnechnik. SPb.: VIR. 2003. 209 s.
  2. D’jakov A.B. Fiziologija podsolnechnika. Krasnodar: VNIIMK. 2004. 76 s.
  3. Sokolov S.Ja., Zamotaev I.P. Spravochnik po lekarstvennym rastenijam (Fitoterapija). 3-e izdanie, stereotipnoe. M.: Medicina. 1990. 464 s.
  4. Nikitina T.I. Lekarstvennye rastenija. Primenenie. Protivopokazanija. Sbory: Spravochnik fitoterapevta. Ufa: Izd-e Bashkirsk. un-ta. 2000. 236 s.
  5. Kiseleva T.L., Karpeev A.A., Smirnova Ju.A., Amalickij V.V., Safonov V.P., Cvetaeva E.V., Blinkov I.L., Kogan L.I., Chepkov V.N., Dronova M.A. Lechebnye svojstva pishhevyh rastenij. Pod obshh. red. prof. T.L. Kiselevoj. M.: Izd-vo FNKJeC TMDL Roszdrava. 2007. 533 s.
  6. Karpenko V.A., Ligaj L.V., Pshukova I.V. Opredelenie soderzhanija inulina v kornjah podsolnechnika. Razrabotka, issledovanie i marketing novoj farmacevticheskoj produkcii: sb. nauch. tr. Pod red. M.V. Gavrilina. Pjatigorsk: Pjatigorskaja GFA. 2011; 66: 106–107.
  7. Pshukova I.V., Konovalov D.A., Karpenko V.A., Ligaj L.V., Kuleshova S.A. Fitohimicheskoe i farmakologicheskoe izuchenie kornej podsolnechnika odnoletnego. Himija rastitel’nogo syr’ja. 2014; 2: 189–194.
  8. Zhurunova M.S., Dautova M.B. Mochekamennaja bolezn’. Zhurnal prikladnyh i fundamental’nyh issledovanij. 2016; 5–6: 977.
  9. Melik-Gusejnov V.V., Gerasimenko S.V. Biologicheski aktivnye veshhestva i jelementnyj sostav kornej podsolnechnika odnoletnego. Voprosy obespechenija kachestva lekarstvennyh sredstv. Moskva. 2013; 1: 24–26.
  10. Melik-Gusejnov V.V., Gerasimenko S.V. Identifikacija fenol’nyh soedinenij v podzemnyh organah podsolnechnika odnoletnego (Heliantus annuus). Vestnik Moskovskogo gosudarstvennogo oblastnogo universiteta. 2013; 3: 34–36.
  11. Melik-Gusejnov V.V., Gerasimenko S.V., Timchenko L.D., Piskov S.I. Izuchenie litoliticheskoj i idiureticheskoj aktivnosti jekstraktov kornja podsolnechnika odnoletnego (Heliantus annuus). Sovremennye problemy nauki i obrazovanija. 2014; 4: 517.
  12. Taova M.R. Issledovanie protivovospalitel’noj aktivnosti izvlechenij list’ev i kornej podsolnechnika maslichnogo. Nauchnoe obozrenie. 2010;1: 24–26.
  13. Musin H.G., Jakupova A.B., Mihajlova E.V., Kuluev B.R. Osobennosti rosta kul’tur geneticheski transformirovannyh (borodatyh) kornej tabaka i vitanii pri izmenenii ob#ema pitatel’noj sredy. Vestnik biotehnologii i fiziko-himicheskoj biologii im. Ju.A. Ovchinnikova. 2017; 13(2): 46–50.
  14. Kuzovkina I.N., Vdovitchenko M.Ju. Geneticheski transformirovannye korni kak model’ izuchenija fiziologicheskih i biohimicheskih processov kornevoj sistemy celogo rastenija. Fiziologija rastenij. 2011;58(5): 787–796.
  15. Dubois M., Gilles K.A., Hamilton J., RobersP.A., Smith F. Colorimetric method for determination of sugars and related substances. Analyt. Chem. 1956; 28: 350–356.
  16. Marinova D., Ribarova F., Atanassova M. Total phenolic and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy. 2005; 40(3): 255–260.
  17. Jakupova A.B., Musin H.G., Bajmuhametova Je.A., Taipova R.M., Kuluev B.R. Indukcija borodatyh kornej podsolnechnika pri pomoshhi shtammov A4 i 15834 Agrobacterium rhizogenes. Biomika. 2018; 10(1): 7–10.
  18. Shashank K., Pandey A.K. Chemistry and Biological Activities of Flavonoids: An Overview. Scientific World Journal [Electronic]. 2013; doi: 10.1155/2013/162750.
  19. Makarenko O.A., Levickij O.A. Fiziologicheskie funkcii flavonoidov v rastenijah. Fiziologija i biohimija kul’turnyh rastenij. 2013; 45(2): 100–112.
  20. Pavlova O.A., Matveeva T.V., Lutova L.A. Rol-geny Agrobacterium rhizogenes. Jekologicheskaja genetika. 2013; 11(1): 59–68.