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EFFECTS OF CULTURAL CONDITIONS ON IAA-PRODUCTIVITY OF ENDOPHYTIC BACTERIAL STRAIN KLEBSIELLA RE3 ISOLATED FROM ROOTS OF CAMELLIA (CAMELLIA sp)

DOI: https://doi.org/10.29296/25877313-2020-11-08
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Issue: 
11
Year: 
2020

Nguyen Van Zhang Ph.D. (Agricul.), Associate Professor, Microbiological Biotechnology Department, Vietnam National University of Agriculture (Vietnam, Hano)i E-mail:nvgiang@vnua.edu.vn Vu Thi Ngoc Diep Junior Research Scientist, Microbiological Biotechnology Department, Vietnam National University of Agriculture (Vietnam, Hanoi) E-mail: vudiep2609@gmail.com Pham Khanh Huyen Junior Research Scientist, Microbiological Biotechnology Department, Vietnam National University of Agriculture (Vietnam, Hanoi) E-mail:phamkhanhhuyen8991@gmail.com E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Department of Genetics, Biotechnology, Breeding and Seed Production, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy (Moscow) E-mail: kalash0407@mail.ru R.N. Kirakosyan Ph.D. (Biol.), Russian State Agrarian University - Moscow Timiryazev Agricultural Academy (Moscow) E-mail: mia41291@mail.ru

Relevance. Camellia (in Vietnam, the camellia is called tea with yellow flowers) is not only used as ordinary tea, but also as a medicinal one. Camel-lia helps prevent tumors, diabetes, reduce cholesterol. Nowadays, research projects with a camellia in Vietnam have mainly focused on the studying of morphological and biological characteristics and selection of camellia. Scientific research in connection with the endophytic microorganisms of ca-mellia was limited. Objective. The purpose of these studies was to assess the effect of cultural conditions and various carbon and nitrogen sources on the IAA producing activity of the strain Klebsiella RE3. Material and methods. The strain Klebsiella RE3 was isolated from the roots of tea shrubs (Camellia sp.) Growing in the gardens of TĐ-GOLDEN TEAVIET (Trà Hoa vàng Tam Đảo), located in Quan Ngoai village, Tam Quan municipality, Tam Dao district (Tam Đảo), Vinh Phúc Province, Vietnam. Results. The research results showed that strain RE3 synthesizes the maximum IAA after 72 hours of cultivation at pH 7 (13.95 μg / ml), temperature (14.28 μg / ml). Conclusion. Of all the tested carbon and nitrogen sources, D-sorbitol and NH4NO3 are the best carbon and nitrogen sources for strain RE3 with IAA production of 12.9 µg / ml and 11.35 µg / ml, respectively.
Keywords: 
Camellia
Klebsiella strain
IАА
productivity
roots
carbon and nitrogen sources
temperature and incubation time

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References: 
  1. Tran Duc Manh, Nguyen Toan Thang, Hoang Thanh Son, et al. Golden Camellias: A Review. Archives of Current Research International. 2019. 16(2): 1–8.
  2. Orel G., Curry A.S. Preliminary morphological assessment of six new, yellow flowering Camellia (Theaceae) species from Viet Nam. In book: International Camellia Journal. 2013; 45.
  3. Tran N., Luong V.D. Camellia Dilinhensis: A New Yellow Species from Viet Nam. In book: International Camellia Journal. 2013;45.
  4. Glickmann E., Dessaux Y. A critical examination of the specificityof the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl. Environ. Microbiol. 1995;61: 793–796.
  5. Panigrahi S., Mohanty S., Rath C. Characterization of endophytic bacteria Enterobacter cloacae G00145 isolated from Ocimum sanctum with Indole Acetic Acid (IAA) production and plant growth promoting capabilities against selected crops. South African Journal of Botany. 2019. https://doi.org/10.1016/j.sajb.2019.09.017.
  6. Mohite B. Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of Soil Science and Plant Nutrition. 2013;13(3): 638–649.
  7. El-Abyad, M. S., El-Sayed, M. A., El-Shanshoury, A.-R., & Farid, M. Optimization of culture conditions for indole-3-pyruvic acid production by Streptomyces griseoflavus. Canadian Journal of Microbiology. 1994; 40(9): 754–760. doi:10.1139/m94-119.
  8. Nguyễn Văn Giang, Trần Thị Đào, Trịnh Thị Thúy An. Phân lập và đánh giá đặc điểm sinh học của một số chủng vi khuẩn nội sinh từ rễ cây nha đam. Tạp chí KH Nông nghiệp Việt Nam. 2016; 14(5): 772–778.
  9. Santi M., KeshabC., Dey S., Pati B.R. Optimization of cultural and nutritional conditions for indole acetic acid production by a Rhizobium sp. isolated from root nodules of Vigna mungo (L.). Hepper. Res. J. Microbiol. 2007; 2: 239–246.
  10. Chandra S., Askari K., Kumaria M. Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth. J. Genet Eng. Biotechnol. 2018; 16(2): 581–586. https://doi.org10.1016/j.jgeb.2018.09.001
  11. Harikrishnan H., Shanmugaiah V., Balasubramanian N. Optimization for production of Indole acetic acid (IAA) by plant growth promoting Streptomyces sp VSMGT1014 isolated from rice rhizosphere. Int. J. Curr. Microbiol. App. Sci. 2014; 3(8): 158–171.
  12. Bharucha U., Patel K., Trivedi U.B. Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard (Brassica nigra). Agricultural Research.2013; 2(3): 215–221. http://dx.doi.org/10.1007/s40003-013-0065-7
  13. Kumari S., Prabha C., Singh A., et al. Optimization of Indole-3-Acetic Acid Production by Diazotrophic B. subtilis DR2 (KP455653), Isolated from Rhizosphere of Eragrostis cynosuroides. International Journal of Pharma Medicine and Biological Sciences. 2018; 7(2): 20–27.
  14. Sridevi M., Yadav N.C.S., Mallaiah K.V. Production of indole acetic acid by Rhizobium isolates from Crolataria species. Res. J. Microbiol. 2008; 3(4): 276–281.
  15. Bhutani N., Maheshwari R., Negi R., Suneja P. Optimization of IAA production by endophytic Bacillus spp. from Vigna radiata for their potential use as plant growth promoters. Israel journal of plant sciences. 2018; 65: 1–2. http://dx.doi.org/10.1163/22238980-00001025
  16. Sasirekha B., Shivakumar S., Sullia S.B. Statistical optimization for improvedindole-3-acetic acid (IAA) production by pseudomonas aeruginosa and demonstration of enhanced plant growth promotion. Journal of Soil Science and Plant Nutrition. 2012; 12: 863–873. https://doi.org/10.4067/S0718-95162012005000038.
  17. Datta C., Basu P. Indole acetic acid production by a Rhizobium species from rootnodules of a leguminous shrub Cajanus cajan. Microbiological Research. 2000; 155: 123–127. https://doi.org/10.1016/S0944-5013(00)80047-6
  18. Mishra V. K., Kumar A. Plant growth promoting and phytostimulatory potential of Bacillus subtilis and Bacillus amyloliquefaciens. ARPN J. Agric. Bio. Sci. 2012; 7(7): 509–518.
  19. Patil N.B., Gajbhiye M., Ahiwale S.S., et al. Optimization of Indole 3-acetic acid (IAA) production by Acetobacter diazotrophicus L1 isolated from Sugarcane. Int. J. Envir. Sci. 2011; 2(1): 295–302.
  20. Garcia-Rodriguez T., Gutierrez-Navarro A.M., Jimenez R.,
  21. Silva J. P. Effects of legume root exudates in indole acetic acid production by Rhizobium meliloti. Pol. J. Soil Sci. 1981;
  22. 14: 45–52