IMMUNOMODULATING PROPERTIES OF THE COMPLEX PLANT SUPPLEMENT «GIPELIV»

DOI: https://doi.org/10.29296/25877313-2020-12-07
Issue: 
12
Year: 
2020

E.A. Ubeeva Post-graduate Student, Banzarov Buryat State University (Ulan-Ude) E-mail: ubeeva.ip@mail.ru V.B. Khobrakova Dr.Sc. (Biol.), Associate Professor, Banzarov Buryat State University; Head of the Laboratory of Experimental Pharmacology, Institutes of General and Experimental Biology at the Siberian Branch of the Russian Academy of Sciences (Ulan-Ude) S.M. Nikolaev Dr.Sc. (Med.), Professor, Banzarov Buryat State University; Principal Researcher of the Laboratory of Experimental Pharmacology, Institutes of General and Experimental Biology at the Siberian Branch of the Russian Academy of Sciences (Ulan-Ude) I.P. Ubeeva Dr.Sc. (Med.), Professor, Banzarov Buryat State University (Ulan-Ude) V.E. Khitrikheev Dr.Sc. (Med.), Professor, Banzarov Buryat State University (Ulan-Ude)

A necessity in finding new hepatoprotective drugs and supplements is dictated by the growing demand: increasingly widespread liver pathologies tend-ing to chronic forms being caused by viruses, toxic agents including some medication. The aim of the work was to determine immunomodulating properties of the complex plant supplement consisting of Hypecoum erectus L., Hedysarum dauricum, Glycyrhhiza uralensis Fisch., Calendula officinalis and Scutellaria baicalensis. The effect of the studied plant supplement was measured by as-sessement of cellular, humoral and macrophageal components of immune response in the mouse model of azathioprine induced immunodeficiency. The experiment was carried out on male F1 line mice (СВАхС57Вl/6). Immunosuppressive agent azathioprine was administered to the control group of animals 50 mg/kg orally once daily during 5 days. The experimental group received same doses of azathioprine and the plant supplement 100 mg/kg once daily for 14 days. The state of the cellular component of the immune response was evaluated by following the standard method of the delayed-type hypersensitivity re-action (DTH) assessment, while the macrophage component was studied in the reaction of phagocytosis of peritoneal macrophages, and the assess-ment of humoral immunity was measured by the number of antibody-forming cells.

Keywords: 
immunomodulating properties
complex plant supplement
cellular immunity
humoral immunity
macrophages

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