CHANGES IN THE LEVEL OF ANTIOXIDANTS IN THE LEAVES OF ROSA MAJALIS AND FRAGARIA VIRIDIS AFTER ACARICIDAL TREATMENT OF FOREST WITH DELTAMETHRIN

DOI: https://doi.org/10.29296/25877313-2020-12-08
Issue: 
12
Year: 
2020

E.A. Chigrinski Ph.D. (Biol.), Omsk State Medical University (Omsk); ORCID: 0000-0002-0844-4090 E-mail: chigrinski@list.ru Gerunova L.K. Dr.Sc. (Veterinary), Professor, Omsk State Agrarian University named after P.A. Stolypin (Omsk); ORCID: 0000-0003-0835-9352 E-mail: gerliud@mail.ru V.D. Konvay Dr.Sc. (Med.), Professor, Omsk State Medical University (Omsk); ORCID: 0000-0002-9082-3507 E-mail: vdconway@bk.ru N.V. Shorin Ph,D. (Agrical.), Associate Professor, Omsk State Agrarian University named after P.A. Stolypin (Omsk) E-mail: nv_shorin@mail.ru P.P. Zolin Ph,D. (Med.), Associate Professor, Omsk State Medical University (Omsk); ORCID: 0000-0003-1900-7117 E-mail: zolin_petr@mail.ru

Relevance. The phytotoxic effects of pesticides are characterized by a decrease in biomass, a slowdown in the growth of the aerial part of plants or their root system, as well as a change in biochemical processes in cells. The effect of synthetic pyrethroids on plants of the Rosaceae family, which includes medicinal plants, has not been deeply studied, and therefore it is of great interest to determine the level of both antioxidant accumulation and other biologically active substances in various parts of plants of this family when exposed to pesticides. Objective. To establish the effect of the synthetic pyrethroid deltamethrin on the level of antioxidants in the leaves of wild rose (Rosa majalis) and green strawberry (Fragaria viridis) during acaricidal forest treatment. Material and methods. R. majalis and F. viridis were the objects for studying. Samples for the study were collected from an experimental forest site with an area of 1 ha at 7, 30 and 60 days after acaricidal treatment of the area with deltamethrin. Alongside, samples were collected at the background site. In the leaves of R. majalis and F. viridis, collected from both sites, the content of ascorbic acid, tocopherol, glutathione, as well as the activity of catalase, superoxide dismutase, and glutathione reductase were determined under laboratory conditions. Results. Deltamethrin causes a decrease in the level of non-enzymatic antioxidants and activation of antioxidant defense enzymes in R. majalis in the early stages after acaricidal treatment of the area. In the late periods of observation, all the studied parameters, with the exception of glutathione re-ductase activity, reached the background level. In the early stages of the effect of deltamethrin on F. viridis, a decrease in the content of non-enzymatic antioxidants in the leaves was observed, and in a later period, inhibition of the activity of antioxidant enzymes was observed against the background of a persisting deficiency of non-enzymatic antioxidants. Conclusion. It was found that acaricidal forest treatment with deltamethrin activates antioxidant enzymes and decreases the content of ascorbate, tocopherol and glutathione in the leaves of R. majalis and F. viridis. These changes are more pronounced in F. viridis.

Keywords: 
antioxidants
synthetic pyrethroids
deltamethrin
Rosa majalis
Fragaria viridis

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