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THE INFLUENCE OF COLD HELIUM PLASMA ON THE STATE OF FREE RADICAL PROCESSES UNDER EXPERIMENTAL THERMAL TRAUMA

DOI: https://doi.org/10.29296/25877313-2021-05-06
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Issue: 
5
Year: 
2021

A.K. Martusevich Dr.Sc. (Biol.), Head of Laboratory of Medical Biophysics, Privolzhsky Research Medical University; Scientific Secretary, Association of Russian Ozone Therapeutists (Nizhny Novgorod, Russia) E-mail: cryst-mart@yandex.ru A.G. Galka Ph.D. (Phys.-Math.), Research Scientist, Privolzhsky Research Medical University; Institute of Applied Physics of the Russian Academy of Sciences Federal Research Center (Nizhny Novgorod, Russia) E.S. Golygina Laboratory Assistant Researcher, Laboratory of Medical Biophysics, Privolzhsky Research Medical University (Nizhny Novgorod, Russia) S.Yu. Krasnova Junior Research Scientist, Laboratory of Medical Biophysics, Privolzhsky Research Medical University (Nizhny Novgorod, Russia) K.L. Belyaeva Laboratory Assistant Researcher, Laboratory of Medical Biophysics, Privolzhsky Research Medical University (Nizhny Novgorod, Russia)

The aim of the study was to estimate the effect of helium cold plasma (5 daily procedures of 1 min) on blood oxidative metabolism of rats with exper-imental thermal trauma. The experiment was performed on 30 male Wistar rats divided into 3 equal-sized groups. The first group of animals (n=10) was intact. The rats of the second and third groups (n=10 in each) were simulated contact thermal burn (area  10% of the body surface) according to our own method, and local treatment of the wound with levomekol was performed. The animals of the third group were additionally treated with a course that included 5 daily procedures for treating a burn wound (1 min) localized on the skin of the back with a stream of cold helium plasma. The distance from the edge of the "plasma torch" to the treated surface was 1.01.2 cm. The cold plasma was produced using a special installation using the principle of microwave-induced ionization of the gas stream. Balloon helium of the A brand was used as the plasma carrier gas. The intensity of pe-roxidation in the plasma and membranes of red blood cells was studied by Fe-induced biochemiluminescence by the light sum of the latter. In addi-tion, the analysis of the biochemiluminescence curve allowed us to calculate the total antioxidant activity of blood plasma. It was found that the course of treatment of an experimental burn wound with cold helium plasma has a positive effect on the blood metabolism of rats, allowing to neutral-ize the manifestations of oxidative stress and exerting an antioxidant effect. It was found that this effect is realized both in blood plasma and in red blood cell membranes.

Keywords: 
cold plasma
blood
lipid peroxidation
thermal trauma

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