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CULTIVATION PARAMETERS AND PHYTOCHEMICAL SCREENING OF PLANTS MARSILEA HIRSUTA R. BR. UNDER IN VITRO CONDITIONS

DOI: https://doi.org/10.29296/25877313-2021-06-08
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Issue: 
6
Year: 
2021

M.V. Sarkisova MSc-student, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy (Moscow) M.Yu. Cherednichenko Ph.D. (Biol.), Associate Professor, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy (Moscow)

Actuality. To preserve natural populations of Marsilea hirsuta it is possible to propose the use of clonal micropropagation, taking into account physio-logical and biochemical characteristics of aseptic plants. Aim. Selection of optimal conditions for in vitro cultivation of M. hirsuta plants and primary phytochemical screening of plant extracts. Material and methods. Isolated explants of M. hirsuta aseptic plants were cultivated on nutrient media with various mineral composition, acidity and consistence. Primary phytochemical screening extracts was carried out according to standard methods. Results. The use of 2-nodal cuttings led to the formation of maximum amount of micro-shoots (7). A decrease in acidity to pH 4 has no significant ef-fect on the efficiency of adventive shoots formation, their morphology and height. At pH 6 or 7, weakened plants with pale green leaves and a poorly developed root system are formed. On the 3rd day on a liquid medium, the plants develop better than on a solid one; by the 7th day, the indicators on both media variants are equalized, after the 10th day, growth on a liquid medium slows down. Primary phytochemical screening of plant extracts showed the presence of components such as tannins and phenolic compounds, flavonoids, alkaloids and proteins. Conclusion. The optimal cutting size is 2 nodes. To obtain the maximum number of nodes and shoot length for the minimum cultivation time, it is recommended to grow M. hirsuta on a liquid nutrient medium (pH 4...5) with a transplant every 10 days. Primary phytochemical analysis showed the presence of some valuable compounds.
Keywords: 
Marsilea hirsuta
in vitro
clonal micropropagation
plant extract
primary phytochemical screening

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References: 
  1. Masatoshi A., Tsuyoshi O. A master of aquatic plants. Nature Aquarium in formation magazine. 2012; 46: 9–38.
  2. Soni P. Marsilea quadrifolia Linn. A valuable culinary and remedi-al fern in Jaduguda, Jjharkhand, India. International Journal of Life Science & Pharma Research. 2012; 2(3): 99–104.
  3. Dihoru G., Dihoru A. Plante rare, periclitateşiendemiceîn Flora României–Listaroşie. Acta Botanica HortiBucurestiensis. 1993–1994: 173–197.
  4. Banciu C., Carasan M.E., Brezeanu A. In vitro propagation of the endangered species Marsilea quadrifolia L. – morphological and biochemical analysis of the regenerates. Romanian Biotechnological Letters. 2009; 14(1): 4139–4145.
  5. Vijayalakshmi M., Kiruthika R., Bharathi K., Ruckmani K. Phyto-chemical screening by LC-MS analysis and in vitro anti-inflammatory activity of Marsilea quadrifolia plant extract. Interna-tional Journal of Pharm Tech Research CODEN (USA). 2015; 8(9):148–157.
  6. Rolli E., Brunoni F., Marieschi M. et al. In vitro micropropagation of the aquatic fern Marsilea quadrifolia L. and genetic stability as-sessment by RAPD markers. Plant Biosystems. 2015; 149(1): 7–14.
  7. Shekhawat M.S., Manokari M. Direct Organogenesis from Rhi-zome Explants in Marsilea quadrifolia L.: A Threatened Fern Spe-cies. Advances in Biology. 2015; 1: 1–6.
  8. Choudhury J., Majumdar S., Roy S., Chakraborty U. Antioxidant activity and phytochemical screening of two edible wetland pterido-phytes Diplazium esculentum (Retz) Sw and Marsilea minuta L.–a comparative study. World journal of pharmaceutical and medical re-search. 2017; 3(9): 195–203.
  9. Aiyegoro O.A., Okoh A.I. Preliminary Phytochemical Screening and In vitro Antioxidant Activities of the Aqueous Extract of Helichry-sum longifolium DC. BMC Complementary and Alternative Medi-cine. 2010; 10(21): 1–8.
  10. Baghel P.S., Ray S. Preliminary phytochemical screening of certain aphrodisiac plants used in traditional system of medicine. Interna-tional Journal of Botany Studies. 2017; 2(5): 33–36.
  11. Kumar A., Ilavarasn R., Jayachandran T. et al. Phytochemical in-vestigation on a tropical plant. Pakistan Journal of Nutrition. 2009; 8: 83–85.
  12. Fehling’s Test– Definition, Principle, Procedure, Result, Uses [Elektronnyj resurs]. URL: https://microbenotes.com-/fehlings-test/ (data obraschenija 09.01.2021).
  13. Biuret Test for Protein [Elektronnyj resurs]. URL: https://microbenotes.com/biuret-test-for-protein/ (data obraschenija 09.01.2021).
  14. Sarkisova M.V., Cherednichenko M.Ju. Otsenka anatomo-morfologicheskih osobennostej rastenij Marsilea hirsuta R. Br. v kul'ture in vitro i ex vitro. Estestvennye i tehnicheskie nauki. 2020; 4(142): 35–38 (Sarkisova M.V., Cherednichenko M.Yu. Ocenka Assessment of anatomical and morphological fea-tures of Marsilea hirsuta R.Br. plants in culture in vitro and ex vitro. Estestvennye i tehnicheskie nauki. 2020; 4(142): 35–38).