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DETERMINATION OF TOTAL TRITERPENE SAPONINS IN A COMBINED DRUG BASED ON JACOB'S LADDER, MOTHERWORT HERBS AND HAWTHORN FRUIT BY SPECTROPHOTOMETRY
DOI: https://doi.org/10.29296/25877313-2021-12-06
Issue:
12
Year:
2021
Purpose: The purpose was to evaluate analytical procedures for the quantitative determination of the total triterpene saponins in a combined herbal medicine based on motherwort herb, hawthorn fruit and jacob's ladder herb by ultraviolet-visible spectrophotometry. The article presents determining the amount of triterpene saponins in a combined sedative drug based on spissum extracts of pharmacopoeial medic-inal plant raw materials of motherwort herb, hawthorn fruits and jacob's ladder herb, using spectrophotometry. In according to requirements of state pharmacopoeia th14, General Pharmacopoeia Monograph.1.2.1.1.0003.15 "Spectrophotometry in the UV and visible regions" using a spectrophotometric method for determining the content of the sum of triterpene saponins in terms of β-escin. The conditions for the analysis were taken from the mono-graph 1.2.5.0039.15 of state pharmacopoeia th14. The method was validated according to requirements of General Pharmacopoeia Monograph 1.1.0012.15 "Validation of analytical methods" in terms of the parameters: specificity, linearity, correctness, repeatability and precision of solutions. The data obtained can be used in the development of regulatory documents for the developed combined drug in the form of a spissum extract.
Keywords:
Herba Polemonii
Polemonium caeruleum L.
Herba Leonuri
Leonurus quinquelobatus Gilib.
Leonurus cardiac L.
Fructus Crataegi
Crataégus spp.
jacob's ladder
herba leonuri
Hawthorn
spissum extract
triterpene saponins
UV-spectrophotometry
References:
- Vincken J.P., Heng L., de Groot A., et al. Saponins, classification and occurrence in the plant kingdom. Phytochemistry. 2017; 68(3): 275–297.
- Mohamed M.A., Ashour A.S. A review on saponins from medicinal plants: chemistry, isolation, and determination. Journal of Nano-medicine Research. 2019; 7: 282-288.
- Vasudeva R.N., Sukhendu B.G., Pushpalatha B., et al. Triterpenoid saponins: a review on biosynthesis, applications and mechanism of their action. Int. J. Pharm. Pharm. Sci. 2015; 7(1): 24–28.
- Van Dyck S., Flammang P., Meriaux C., Bonnel D., Salzet M., Fournier I., et al. Localization of secondary metabolites in marine invertebrates: contribution of MALDI MSI for the study of sapo-nins in cuvierian tubules of H. forskali. PLoS One. 2019; 5: e13923
- Rao A.V., Gurfinkel D.M. The bioactivity of saponins: triterpenoid and steroidal glycosides. Drug Metabol Drug Interact. 2020; 17(1–4): 211–235. This review summarizes the saponins chemistry, me-tabolism and in role in different diseases.
- Challinor V.L., De Voss J.J. Open-chain steroidal glycosides, a di-verse class of plant saponins. Nat Prod Rep. 2019; 30(3): 429–454.
- Bruneton J. Pharmacognosy, Phytochemistry, Medicinal Plants. Lavoisier Publishing, Paris. 2017; 538–544 (ISBN 2-4730-0028-7).
- Project Summary: Functional Genomics of Triterpene Saponin Bio-synthesis in Medicago Truncatula. http://www.noble.org/Plantbio/Dixon/Personnel/projectsummaries/Saponin.html.
- Gosudarstvennaja farmakopeja Rossijskoj Federatsii XIV. Tom II. Ministerstvo zdravoohranenija Rossijskoj Federatsii. 2018; 1980-1986 (Gosudarstvennoj farmakopei Rossijskoj Feder-acii XIV. Tom II. Ministerstvo zdravoohranenija Rossijskoj Feder-acii. 2018; 1980-1986).