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STUDY OF THE STABILITY OF 2,6-DI-TERT-BUTYL-4-METHYLHYDROXYBENZENE IN BIOLOGICAL MATERIAL

DOI: https://doi.org/10.29296/25877313-2022-01-03
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Issue: 
1
Year: 
2022

A.P. Chernova Ph.D. (Chem.), Associate Professor, National Research Tomsk Polytechnic University (Tomsk, Russia) E-mail: apa2004@mail.ru; https://orcid.org/0000-0001-7002-492X M.K. Elizarova Ph.D. (Pharm.), Yeisk Medical College of the Ministry of Health of the Krasnodar Territory (Yeisk, Russia) E-mail: goukkemk@mail.ru; https://orcid.org/0000-0002-8944-4358 V.K. Shormanov Dr.Sc. (Pharm.), Professor, Kursk State Medical University (Kursk, Russia) E-mail: R-WLADIMIR@yandex.ru; https://orcid.org/0000-0001-8872-0691 O.I. Pugacheva Post-graduate Student, Kursk State Medical University (Kursk, Russia) E-mail: lnpugacheva@yandex.ru; https://orcid.org/0000-0002-2074-0423

Aim purpose. Study of stability of 2,6-di-tert-butyl-4-methylhydroxybenzene in biological material. Methods. The studies were carried out by GC-MS, TLC and UV spectrophotometry.2,6-di-tert-butyl-4-methylhydroxybenzene was isolated from the bi-omatrix (liver tissue) by infusion with a mixture of ethyl acetate-acetone (7:3). The analyte was purified by combining extraction (water-ethyl acetate system) and semi-preparative column chromatography (sorbent – silica gel L 40/100 μm, eluent – hexane-acetone (9.5:0.5)), Results. It was found that at –22оС, 2оС, 10оС, 20оС and 30оС. 2,6-di-tert-butyl-4-methylhydroxybenzene remains in the biomaterial for 469, 371, 315, 301 and 259 days, respectively. The possibility of mathematical description of the dynamics of analyte decomposition in biomaterial at the indicated temperatures using the hyperbole equation has been studied. Coefficients in the equation of hyperbola (km), Calculated from the results of the experi-ment, for temperatures of –22оС, 2оС, 10оС, 20оС and 30оС were respectively 11415, 7508, 4983, 3696 and 2156. A linear dependence of km was found. from the storage temperature tо, which is described by the equation: km = 182,24(50–tо) –1702,36. Conclusion. The dynamics of decomposition of 2,6-di-tert-butyl-4-methylhydroxybenzene in the liver tissue at –22оС – + 30оС, which can be de-scribed by the hyperbole equation, is shown. The possibility of predicting the nature of analyte stability in biomaterial in the specified temperature range is shown.
Keywords: 
2
6-di-tert-butyl-4-methylhydroxybenzene
biological material
storability
dynamics of decomposition
prediction of stability

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