CHARACTERISTICS OF ENDOPHYTIC BACTERIA, ISOLATED FROM THE ROOTS OF IPOMOEA PES-CAPRAE (L.)

DOI: https://doi.org/10.29296/25877313-2022-01-04
Issue: 
1
Year: 
2022

Nguyen Van Giang Associate Professor, Lecturer of the Microbial Department, Faculty of Biotechnology, Vietnam National University of agriculture (Ha Noi, Viet Nam) E-mail: nvgiang@vnua.edu.vn Nguyen Thi Thu Mc.S. of the Microbial Department, Faculty of Biotechnology, Vietnam National University of Agriculture (Ha Noi, Viet Nam) E-mail: thunguyenntc@gmail.com Vu Thi Linh Mc.S. of the Microbial Department, Faculty of Biotechnology, Vietnam National University of Agriculture (Ha Noi, Viet Nam) E-mail: linhvt030899@gmail.com E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Head of Department of Biotechnology, Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazeva (Moscow, Russia) E-mail: kalash0407@mail.ru R.N. Kirakosyan Ph.D. (Biol.), Associate Professor, Head of Department of Biotechnology, Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazeva (Moscow, Russia) E-mail: mia41291@mail.ru

Relevance. Endophytic bacteria live in the internal tissues of healthy plants and do not cause any harm to the host plant. They positively affect plants' growth and development and help them absorb nutrients from the environment. Endophytic bacteria protect plants from various phytopatho-gens due to the synthesis of phytohormones and contribute to converting insoluble mineral salts into a form accessible to plants. Studies on endophyt-ic bacteria isolated from I.pes-caprae in Vietnam are not yet numerous. Purpose of the study. Isolate and characterize endophytic bacteria from the roots of Ipomoea pes-caprae. Identify promising strains. Material and methods. The objects of the study were I. pes-caprae roots isolated from plants collected in Thang Hai village, Tinh Hai commune, Tinh Gia district, Thanh Hoa province. Before isolating endophytic bacteria strains, root samples were thoroughly washed with distilled water and steri-lized with mercuric chloride solution for 5 minutes. The homogenate was cultivated on Luria-Bertani nutrient medium. The IAA concentration from bac-terial strains was determined by the method of Glickmann and Dessaux (1995). A quantitative determination of phosphate-dissolving activity was car-ried out by measuring the phosphate accumulation in the solution in which the strains under study were cultivated. Results. Fifteen strains of endophytic IAA-producing bacteria were isolated on LB nutrient medium supplemented with L-tryptophan. Three (TH10R, TH11T, and TH13T) strains with high IAA synthesizing ability (from 2.53 to 4.19 μg / ml) were selected. The phosphate-dissolving activity of these iso-lates ranged from 1.06 mg / L to 3.64 mg / L. The TH10R strain was superior to other strains in its ability to synthesize IAA and phosphate solubility and was identified as Bacilus mycoides and named Bacilus mycoides TH10R.

Keywords: 
Ipomea pes-caprae
siderophore
phosphate solubilizing
IAA production

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