INVESTIGATION AND VALIDATION OF QUANTITATIVE ANALYSIS OF PHENOLIC COMPOUNDS AND CHLOROGENIC ACID IN THE VACCINIUM ULIGINOSUM LEAVES

DOI: https://doi.org/10.29296/25877313-2022-02-03
Issue: 
2
Year: 
2022

A.A. Shamilov Ph.D. (Pharm.), Associate Professor of Department of Pharmacognosy, Botany and Technology of Phytopreparations, Pyatigorsk Medical-Pharmaceutical Institute (PMPI), Branch of Volgograd State Medical University, Ministry of Health of Russia (Pyatigorsk, Russia) E-mail: shamilovxii@yandex.ru, ORCID iD 0000-0002-6730-9518 V.N. Bubenchikova Dr.Sc. (Pharm.), Professor, Head of Department of Pharmacognosy and Botany, Kursk State Medical University, Ministry of Health of Russia (Kursk, Russia) E-mail: bubenhikova.ksmu@yandex.ru; ORCID iD0000-0001-9682-0684 E.R. Garsiya Lecturer, Department of Pharmacognosy, Botany and Technology of Phytopreparations, Department of Inorganic, Physical and Colloidal Chemistry, Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University, Ministry of Health of Russia (Pyatigorsk, Russia) E-mail: x-pharm@mail.ru, ORCID iD 0000-0003-3217-0680 Kh.A. Ibaeva Post-graduate Student, Department of Pharmacognosy, Botany and Technology of Phytopreparations, Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University, Ministry of Health of Russia (Pyatigorsk, Russia) E-mail: ibaeva.hadizhat@mail.ru M.V. Larsky Ph.D. (Pharm.), Head of Department of Pharmaceutical Chemistry, Pyatigorsk Medical-Pharmaceutical Institute, Branch of Volgograd State Medical University, Ministry of Health of Russia (Pyatigorsk, Russia) E-mail: m.v.larsky@pmedpharm.ru; ORCID iD0000-0002-4406-7165

Relevance. The modern requirements of qualitative control of medicinal plant raw material recommends to evaluate total amount of biological active compounds and amount of major substances. Aim. Investigation and validation of quantitative analysis of total amount of phenolic compounds and major substance chlorogenic acid in the Vaccini-um uliginosum leaves. Materials and methods. Quantitative analysis of total amount of phenolic compounds in the V. uliginosum leaves was carried out by spectropho-tometry. Amount of chlorogenic acid was measured by capillary electrophoresis as main method and by high-performance liquid chromatography as comparative method. Developed methods were validated. Results. We investigated and validated methods of spectrophotometric analysis of total amount of phenolic compounds and quantitative analysis of chlorogenic acid by capillary electrophoresis. Total amount of phenolic compounds were 4.94±0.17% – 9,09±0,17%, amount of chlorogenic acid – 1.75±0.08% – 4.34±0.16% Conclusions. Medicinal plant raw material – Vaccinium uliginosum leaves –are recommended to be standardized by total amount of phenolic com-pounds and amount of major substance – chlorogenic acid - using spectrophotometry and capillary electrophoresis respectively. Total amount of phe-nolic compounds calculated to g chlorogenic acid at 100 g of raw material should be no less 4.5%, amount of chlorogenic acid – no less 1.5%.

Keywords: 
Vaccinium uliginosum
leaves
phenolic compounds
chlorogenic acid
spectrophotometry
capillary electrophoresis

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