ESTIMATION OF ENZYME CATALYTIC ACTIVITY IN BIOPTATES OF LOWER LIMB VARICOSE DEFORMED VEINS DEPENDING ON THE SEVERITY OF CHRONIC VENOUS INSUFFICIENCY

DOI: https://doi.org/10.29296/25877313-2022-02-07
Issue: 
2
Year: 
2022

A.V. Minaev Lecturer, Department of Biological Chemistry, Moscow State University of Medicine and Dentistry. A.I. Evdokimova (Moscow, Russia) E-mail: minaev-msmsu@mail.ru T.P. Vavilova Dr. Sc. (Med.), Professor, Department of Biological Chemistry, Moscow State University of Medicine and Dentistry. A.I. Evdokimova (Moscow, Russia) E-mail: tpvavilova@rambler.ru G.I. Alekberova Ph.D. (Med.), Senior Lecturer, Department of Biological Chemistry, Moscow State University of Medicine and Dentistry. A.I. Evdokimova (Moscow, Russia) Е-mail: oliwka1987@mail.ru G.F. Yamaletdinova Ph.D. (Biol.), Senior Lecturer, Department of Biological Chemistry, Moscow State University of Medicine and Dentistry. A.I. Evdokimova (Moscow, Russia) E-mail: yamaletdinova.g@yandex.ru Kh.A. Abduvosidov Ph.D. (Med.), Associate Professor, Department of Human Morphology, Moscow State University of Medicine and Dentistry. A.I. Evdokimova (Moscow, Russia) Е-mail: sogdiana99@gmail.com

Relevance. Varicose veins of the lower extremities are recognized as one of the most common pathologies of the venous system of the lower ex-tremities. Along with various involutive and morphological changes occurring in the venous wall, changes at the molecular level play a key role in path-ogenesis. The content of various biologically active substances directly in the varicose vein wall, reflecting the speed of biochemical processes, has not been practically studied. Aim. To study the catalytic activity of lactate dehydrogenase and creatine kinase in biopsy specimens of the vein wall in patients with varicose dis-ease of the lower extremities of different classes of chronic venous insufficiency according to the CEAP classification. Matherials and Methods. In the experiment, tissue of the venous wall taken from 141 patients with varicose disease of the lower extremities of classes C2-C6 (according to the CEAP classification) was studied. All examined patients were divided into 4 groups depending on the severity of the disease. Determination of enzyme activity was performed by spectrophotometric method. Results. An increase in the activity of lactate dehydrogenase and a decrease in the activity of creatine kinase were revealed as the disease pro-gressed. A positive correlation was found between lactate dehydrogenose activity and the class of chronic venous insufficiency and a negative correla-tion between creatine kinase activity and the class of chronic venous insufficiency. Conclusions. An increase in the catalytic activity of lactate dehydrogenase and a decrease in the activity of creatine kinase in biopsies of varicose veins of the lower extremities are directly related to the progression of the disease, which is explained by the predominance of anaerobic processes over aerobic ones in the varicose deformed venous wall, an increase in tissue hypoxia and a decrease in energy supply.

Keywords: 
varicose veins
lactate dehydrogenase
creatine kinase
chronic venous insufficiency

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