CHANGES IN THE ACTIVITY OF LYSOSOMAL CYSTEINE PROTEASES OF SPERMOPLASM IN PATIENTS WITH IMPAIRED REPRODUCTIVE FUNCTION

DOI: https://doi.org/10.29296/25877313-2022-04-04
Issue: 
4
Year: 
2022

A.F. Ishtulin
Assistant, the Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics,
the Faculty of Additional Professional Education,
Ryazan State Medical University named after I. P. Pavlov, (Ryazan, Russian Federation)
E-mail: a.ishtulin@yandex.ru
ORCID ID: 0000-0001-9750-1642
N.V. Korotkova
Ph.D. (Med.), Associate Professor, the Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics,
the Faculty of Additional Professional Education;
Senior Research Scientist, the Central Research Laboratory,
Ryazan State Medical University named after I. P. Pavlov (Ryazan, Russian Federation)
E-mail: fnv8@yandex.ru
ORCID ID: 0000-0001-7974-2450
I.V. Matveeva
Ph.D. (Med.), Associate Professor, Head of the Department of Biological Chemistry with the Course of Clinical Laboratory Diagnostics, the Faculty of Ad-ditional Professional Education,
Ryazan State Medical University named after I. P. Pavlov (Ryazan, Russian Federation)
E-mail: i.matveeva@rzgmu.ru
S.A. Isakov
Dr.Sc. (Med.), Professor, the Department of Dermatovenerology,
Ryazan State Medical University named after I. P. Pavlov (Ryazan, Russian Federation)
I.V. Minaev
Student, Faculty of General Medicine,
Ryazan State Medical University named after I. P. Pavlov (Ryazan, Russian Federation)
E-mail: ivan.minarv98@yandex.ru
ORCID ID: 0000-0003-0728-8708
P.M. Polyakova
Student, Faculty of General Medicine,
Ryazan State Medical University named after I. P. Pavlov (Ryazan, Russian Federation)
E-mail: pelageia18@gmail.com
ORCID ID: 0000-0002-0681-8183

Relevance. One of the serious problems of modern society is male infertility, which accounts for 7% of the total structure of infertile marriages. The reasons leading to it are not always clear. All researches of ejaculate are mainly based on chemical microscopic explorations. The biochemical indica-tors of sperm are currently being determined in insufficient volume, therefore the introduction of new biochemical markers for the diagnosis of de-creased fertility in men is very relevant. Object. To examine the activity of cathepsins B, L and H contained in the acrosome of spermatozoa among men with impaired reproductive function. Materials and methods. The material for the investigation was spermoplasm. Spermoplasm was obtained from 30 patients diagnosed with chronic prostatitis and 30 patients diagnosed with varicocele with complaints of reproductive health. The control group consisted of 30 patients without diseas-es of the genitourinary system during physical examination and without complaints in anamnesis about reproductive health. Spermoplasm was ob-tained by centrifugation of a sperm sample for 10 minutes at a speed of 1000 rpm. The activity of cathepsins B, L, H in the spermoplasm was deter-mined by the spectrofluorimetric method according to A.J. Barrett and H. Kirschke. Results. The activity of cathepsin B among patients diagnosed with chronic prostatitis was reduced by 3.4 times, in patients diagnosed with varico-cele by 18.5 times. The activity of cathepsin L among patients with chronic prostatitis is reduced by 3.5 times, with varicocele by 20 times. The activi-ty of cathepsin H in chronic prostatitis decreases by 3.3 times, in patients with varicocele by 25.5 times. Conclusions. The activity of lysosomal cysteine proteases B, L, H among patients with impaired reproductive function was explored. A decrease in the activity of cathepsins B, L, H was found among these patients, as distinct from the patients without signs of fertility disorders. In this way, chang-ing the activity of cathepsins B, L and H can be considered as one of the possible markers for the diagnostics of male infertility.

Keywords: 
proteolysis
chronic prostatitis
varicocele
cysteine cathepsins
spermoplasm.

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