ASSESSMENT OF THE SELECTINS AND THEIR LIGAND PSGL-1 LEVEL IN PATIENTS WITH ACUTE VENOUS THROMBOSIS

DOI: https://doi.org/10.29296/25877313-2022-05-04
Issue: 
5
Year: 
2022

N.V. Korotkova
Ph.D. (Med.), Associate Professor, Department of Biological Chemistry with Course
of Clinical Laboratory Diagnostics of Continuing Professional Education Faculty,
Senior Research Scientist at the Central Research Laboratory, Ryazan State Medical University (Ryazan, Russia)
E-mail: fnv8@yandex.ru
R.E. Kalinin
Dr.Sc. (Med.), Professor, Head of the Department of Cardiovascular, Endovascular Surgery and Diagnostic Radiology,
Ryazan State Medical University (Ryazan, Russia)
E-mail: kalinin-re@yandex.ru
I.A. Suchkov
Dr.Sc. (Med.), Professor, the Department of Cardiovascular, Endovascular Surgery and Diagnostic Radiology,
Ryazan State Medical University (Ryazan, Russia)
E-mail: i.suchkov@rzgmu.ru
N.D. Mzhavanadze
Ph.D. (Med.), Associate Professor, the Department of Cardiovascular, Endovascular Surgery and Diagnostic Radiology,
Senior Research Scientist ae the Central Research Laboratory, Ryazan State Medical University (Ryazan, Russia)
E-mail: nina_mzhavanadze@mail.ru
A.A. Nikiforov
Ph.D. (Med.), Associate Professor in the Department of Pharmacology with Course of Pharmacy
of Continuing Professional Education Faculty, Head of the Central Research Laboratory,
Ryazan State Medical University (Ryazan, Russia)
E-mail: alnik003@yandex.ru
B.K. Romanov
Dr.Sc. (Med.), Head of the Department of Pharmacology, the Pediatric Faculty,
Russian National Research Medical University named after N.I. Pirogov (Moscow, Russia)
O.V. Bodrova
Student, the Faculty of Medicine,
Ryazan State Medical University (Ryazan, Russia)
E-mail: bodrowa.olga2015@yandex.ru

Relevance. Currently, the violation of the adhesive function of the endothelium is considered one of the components of endothelial dysfunction, and therefore is in the center of attention of doctors of various specialties. Violation of the adhesive function is manifested by a change in the synthesis of cell adhesion molecules: hyper- or hypo-expression of the latter leads to a change in the adhesion cascade of leukocytes and disruption of their interactions with endotheliocytes. Aim. To evaluate the concentration of cell adhesion molecules – selectins P, E, L and their glycoprotein ligand in the blood serum of patients with acute venous thrombosis. Materials and Methods. The study included 20 patients whith acute venous thrombosis and 12 subjects were used as control (healthy volunteers). The participants of the study were comparable in age and gender. Serum samples were isolated from peripheral venous whole blood samples. Quantitative measurement of P-selectin and its glycoprotein ligand PSGL-1 was performed using the Sandwich Enzyme-Linked ImmunoSorbent Assay (ELISA) with ELISA Kit for Selectin, Platelet (SELP) (catalogue № SEA569Hu), PSGL-1 ELISA kit (catalogue № SEA985Hu), and Stat Fax 2100 analyzer (microplate reader) (Awareness technology Inc. PalmCity, FL 34990, USA). Results. The concentration of selectin P in peripheral blood in patients with acute venous thrombosis statistically significant decrease by 2.5 times was discovered. The concentration of selectin E showed a downward trend, but it was not statistically significant, which may indicate a lesser degree of involvement of Е selectin in the pathogenesis of venous thrombosis. The concentration of selectin L tended to increase. Concentrations of the glycoprotein ligand of selectins PSGL-1 were statistically significantly increased in the peripheral blood of patients with acute venous thrombosis. Conclusions. Acute venous thrombosis is accompanied by a statistically significant decrease in the concentration of P-selectin, a tendency to decrease in E-selectin, an increase in the concentrations of L selectin and the ligand of selectins PSGL-1 in the blood serum.

Keywords: 
adhesive function of the endothelium
selectins P
E
L
venous thrombosis
glycoprotein ligand of selectins PSGL-1.

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