ANTIMICROBIAL AND ANTIMYCOTIC ACTIVITY OF BONE MATRIX IMPREGNATED WITH A SOLUTION OF SANGUIRITRIN AFTER STERILIZATION WITH AN OZONE-OXYGEN MIXTURE

Relevance. Currently, the problem of creating implantation materials based on biodegradable polymers of natural origin with specified properties con-tinues to be relevant. Аim. To evaluate the antimicrobial properties of bone matrix samples impregnated with sanguiritrin solution after sterilization with an ozone-oxygen mixture. Material and methods. Cylindrical samples of cortical bone and bone matrix impregnated with sanguiritrin substance, a medicinal product of plant origin, were used in the work. The samples were sterilized with an ozone-oxygen mixture with an ozone concentration of 8 mg/l. Antimicrobial activity was tested using bacterial strains: Staphylococcus aureus, Escherichia coli; strains of micromycetes: Aspergillus niger, Cladosporium herbarum. Results. The study found that there was no significant effect of ozone-oxygen sterilization with an ozone concentration of 8 mg/l and a duration of 15 minutes in the flow mode on the antibacterial and antimycotic properties of the studied bone samples impregnated with a solution of antimicrobial and antifungal medicinal product of plant origin – sanguiritrin. Sanguiritrin alkaloids released after sterilization from demineralized and disorganized bone ma-trix do not lose their antimicrobial and antimycotic properties against gram-positive and gram-negative bacteria, as well as micromycetes. Conclusion. The approach proposed in the study can be used to further improve and develop new implantation drugs with specified antimicrobial properties and methods of their sterilization.