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ANATOMICAL AND DIAGNOSTIC FEATURES AND QUALITY INDICATORS OF HAPLOPHYLLUM DAURICUM (L.) G. DON

DOI: https://doi.org/10.29296/25877313-2023-01-05
Issue: 
1
Year: 
2023

A.V. Polonova
Post-graduate Student, Junior Researcher, Laboratory of Physiologically Active Substances and Phyto-engineering,
Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences (Ulan-Ude, Russia)
E-mail: nv.polonova@gmail.com
V.V. Taraskin
Ph.D. (Pharm.), Head of Laboratory of Chemistry of Natural Systems,
Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences (Ulan-Ude, Russia)
Zh.A. Tykheev
Ph.D. (Pharm.), Head of Laboratory of Physiologically Active Substances and Phyto-engineering,
Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences (Ulan-Ude, Russia)

Aim of the research – determination of anatomical and diagnostic features and main quality indicators of Haplophyllum dauricum of Buryatian flora. Material and methods. The objects of the study were the herb and roots of Haplophyllum dauricum, growing within the Dzhidinsky district of the Re-public of Buryatia. They were collected during the fruiting period (July 2020). The determination of anatomical and diagnostic features and quality indica-tors were carried out in accordance with the methods described in the State Pharmacopoeia of the XIV Edition. Micropreparations were taken on an op-tical microscope with an imaging system «μ-Vizor». Results. Diagnostic features in the microscopic structure of the leaf blade, stem, petal and root were determined. It was revealed that 70% ethanol was the most optimal extraction solvent for both aerial and underground parts of the plant, where it showed the highest yield of extractives. The norms of quality indicators (humidity; total ash; ash insoluble in hydrochloric acid; extractive substances extracted by different solvents: purified water, 70% ethanol) for Haplophyllum dauricum herb and roots were established. Conclusion. The main anatomical and diagnostic features and quality indicators of the aerial and underground parts of Haplophyllum dauricum, as a source of lignans with antitumor activity, were determined.

Keywords: 
Haplophyllum dauricum
Haplophyllum
microscopic analysis
quality indicators.

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