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CHANGES IN THE ACTIVITY OF LYSOSOMAL CYSTEINE PROTEASES IN VASCULAR WALL HOMOGENATES IN VARICOSE VEINS OF THE LOWER EXTREMITIES

DOI: https://doi.org/10.29296/25877313-2023-04-08
Issue: 
4
Year: 
2023

M.G. Konopleva
Senior Laboratory Assistant-Researcher, Central Research Laboratory, Ryazan State Medical University (Ryazan, Russia)
E-mail: mari.konopleva.97@mail.ru
Z.S. Gazaryan
Orthopedic Traumatologist, GBU RO OKB (Ryazan, Russia)
E-mail: GhazaryanZ@yandex.ru
R.E. Kalinin
Dr.Sc. (Med.), Professor, Head of Department of Cardiovascular, X-Ray Endovascular Surgery and Radiation Diagnostics, Ryazan State Medical University (Ryazan, Russia)
E-mail: kalinin-re@yandex.ru
I.A. Suchkov
Dr.Sc. (Med.), Professor, Department of Cardiovascular, X-Ray Endovascular Surgery and Radiation Diagnostics, Ryazan State Medical University (Rya-zan, Russia)
E-mail: i.suchkov@rzgmu.ru
N.V. Korotkova
Ph.D. (Med.), Associate Professor, Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics, Continuing Professional Education Faculty, Senior Researcher, Central Research Laboratory, Ryazan State Medical University (Ryazan, Russia)
E-mail: fnv8@yandex.ru

Relevance. The article deals with the issue of changes in the activity of lysosomal cysteine proteases – cathepsins L, B, H in homogenates of vascu-lar wall, obtained from patients with varicose veins of the lower extremities. Due to the fact that the proteolytic processes occurring in the wall of vari-cose transformed veins currently remain poorly understood, and the incidence of varicose veins is increasing, the relevance of the topic remains at a high level. Materials and methods. The study shows the results from 22 patients divided into two groups: the first group (n=12): patients with varicose veins of the lower extremities, the second group (control) – patients who had healthy vein sections, taken during femoral-popliteal bypass surgery using au-tovena (n=10). The material for the study was homogenates of vascular wall, obtained during surgical intervention. The vessels were weighed on elec-tronic scales (Ohaus corporation, USA). The samples were crushed and placed in a glass of the Potter S homogenizer (Sartorius, Germany). Homoge-nized for 60 seconds with a rotation speed of a Teflon pestle of 1500 rpm. The activity of cathepsins B, L and H was determined spectrofluorimetrically on the RF – Zhimadzu Spectrofluorometer (Japan). The protein content was assessed using the Lowry method by the commercial set of the NPC "Eco-service" (St. Petersburg). Results. The activity of cathepsin B in the homogenate of varicose veins was increased by 35 times compared to the control group. The activity of cathepsin D in the homogenate of varicose veins was also increased 17-fold compared to the control. The activity of cathepsin H in varicose vein ho-mogenate showed a unidirectional trend - it was higher than the control values, but by a smaller number of times – by 9. Thus, in varicose vein ho-mogenates, all three studied cathepsins increase their activity, but to varying degrees. The greatest changes, according to our study, confirmed the activity of cathepsin B. Conclusions. Varicose veins of the lower extremities are accompanied by a significant increase in the activity of lysosomal cysteine proteinases – cathepsins B, L and H in the wall homogenates of the affected vein, which may indicate the involvement of LC in the pathogenesis of venous trans-formation.
Keywords: 
lysosomal cysteine proteinases
cathepsin B
cathepsin L
cathepsin H
varicose veins of the lower extremities
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