PHYTO-STIMULATING EFFECTS OF PARACETAMOL BIODESTRUCTION PRODUCT ON URTICA DIOICA

Relevance. The search for environmentally friendly methods of processing pharmaceutical waste to obtain new biologically active compounds on their basis, including phytostimulating action, is especially relevant in recent years due to global pharmaceutical pollution of water bodies in many countries. The results of previous studies have shown that the product of bacterial degradation of paracetamol exhibits pronounced phytostimulating properties in relation to medicinal plants of Plantaginaceae, Asteraceae, Linaceae, Lamiaceae and other families. The purpose of this study is to study the effect of PBP on morphometric parameters, dry biomass and the content of biologically active substanc-es in the leaves of stinging nettle (Urtica dioica L.), a medicinal plant of the family. nettles (Urticaceae). Material and methods. We used bacterial degradation of paracetamol obtained on the basis of the Laboratory of Alkanotrophic Microorganisms, PFRC, Ural Branch, Russian Academy of Sciences (Perm). Stinging nettle leaves collected from plants treated with bacterial degradation of paracetamol were used to determine the biomass by gravimetric method, morphometric parameters, as well as the content of the total free organic acids, hy-droxycinnamic acids and ascorbic acid by titrimetric analysis and spectrophotometry. Water and alcohol extracts from medicinal plant materials were obtained according to the method of the Global Fund XIV ed. Results. Under the influence of bacterial degradation of paracetamol, the morphometric parameters – the length and width of the nettle leaves in-crease by 115 % and 66 %, respectively, the total collection of leaf biomass increases by 40 % compared to the control (water). The increase in the content of the total free organic acids under the influence of bacterial degradation of paracetamol is 18.5%, hydroxycinnamic acids – 6.8%, ascorbic acid – 12.5%. Conclusion. The product of bacterial degradation of paracetamol has a obvious phytostimulating effect on stinging nettle. Conflict of interests. The authors declare the absence of obvious and potential conflicts of interest related to the publication of this article. Contribution of authors. E.V. Vikhareva– planning an experiment, writing an article; E.V. Slabinskaya, I.I. Mishenina, D.F. Rubtsov– conducting field and laboratory experiments, processing the results; M.I. Rychkova– obtaining a biodegradation product of paracetamol. Source of funding. The researches were carried out using the equipment of the Regional Profiled collection of alkanotrophic Microorganisms CCP Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences according to the state task AAAA19-119112290008-4 and with the financial support of the Perm Scientific and Educational Center "Rational Subsoil Use", 2022.