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CORRELATION OF THE COMPONENT COMPOSITION OF HUMIC ACIDS OF PELOIDS WITH THEIR POLYMODAL ACTION

DOI: https://doi.org/10.29296/25877313-2023-07-02
Issue: 
7
Year: 
2023

Y.V. Zhernov
Dr.Sc. (Med.), Associate Professor, Professor of the Department of General Hygiene,
Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
(Sechenov University) (Moscow, Russia)
M.A. Krivopalova
Ph.D. (Chem.), Associate Professor, Associate Professor of the Department of Medical Chemistry,
FSBEI HE "Samara State Medical University" of the Ministry of Health of Russia (Samara, Russia)
P.G. Mizina
Dr.Sc. (Pharm.), Professor, Advisor,
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)
N.P. Avvakumova
Dr.Sc. (Biol.), Professor, Professor of the Department of Medicinal Chemistry,
FSBEI HE "Samara State Medical University" of the Ministry of Health of Russia (Samara, Russia)
A.V. Zhdanova
Ph.D. (Pharm.), Associate Professor of the Department of Medicinal Chemistry,
FSBEI HE "Samara State Medical University" of the Ministry of Health of Russia (Samara, Russia)
E-mail: a.v.zhdanova@samsmu.ru
A.V. Sevastyanova
Post-Graduate Student, the Department of Medical Chemistry,
FSBEI HE "Samara State Medical University" of the Ministry of Health of Russia (Samara, Russia)

Relevance. The source of humic substances and the method of isolation determines their structure, the specificity of binding of individual fragments, the degree of condensation and conjugation, molecular weights and other characteristics. Humic acids of peloids have a high pharmacological activity due to the presence of various functionals in their composition, which, along with a high molecular weight, allows them to be attributed to the group of polyelectrolytes and determines the universality of the effect. The aim of the work was to identify the components of humic acid hydrolysate of low-mineralized silt sulfide mud to substantiate their polymodal action. Material and methods. The object of the study was a solution obtained by extraction into hexane, products of acid hydrolysis of humic acids. The obtained samples were studied using the following methods: chromatography-mass spectrometry (GC-MS) on an Agilent 7890 A chromatograph, IR spectroscopy on a Perkin-Elmer System - 2000 spectrophotometer, elemental quantitative analysis on a Vario EL analyzer. Results. GC-MS analysis of humic acid hydrolysis products showed the presence of acidic components in the sample and includes both low- and high-molecular fatty monobasic and dibasic acids of saturated unsaturated type. The presence of phenolic derivatives of acrylic acid in the composition causes the inhibitory effect of HA on cell proliferation. The neutral fraction is represented by high-molecular alcohols of natural origin. Conclusion. Thus, the above components of the hydrolysate justify the currently established and potentially possible effects of humic acids of peloids.
Keywords: 
humic acids
hydrolyzate
components.
References: 
  1. Buzlama A.V., Chernov Ju.N. Perspektivy ispol'zovanija solej guminovyh kislot dlja razrabotki novyh lekarstvennyh preparatov. V sbornike: Puti i formy sovershenstvovanija farmacevticheskogo obrazovanija. Sozdanie novyh fiziologicheski aktivnyh veshhestv. Materialy 6-j Mezhdunarodnoj nauchno-metodicheskoj konferencii «Farmobrazovanie-2016». FGBOU VO "Voronezhskij gosudarstvennyj universitet". 2016: 196–198.
  2. Perminova I.V., Hatfield K. Remediation chemistry of humic substances: theory and implications for technology. Use of Humic Substances to Remediate Polluted Environments: From Theory to Practice, NATO Science Series IV: Earth and Environmental Sciences 52, Springer, Dordrecht, The Netherlands. 2005; 1: 3–36.
  3. Avvakumova N.P., Krivopalova M.A., Glubokova M.N., Katunina E.E., Zhdanova A.V. Poluchenie i svojstva nizkomineralizovannyh ful'vovyh kislot peloidov. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2020; 23(5): 3–9.
  4. Logvinova L.A., Zykova M.V., Krivoshhekov S.V., Drygunova L.A., Perederina I.A., Golubina O.A., Perminova I.V., Konstantinov A.I., Belousov M.V. Sravnitel'noe farmakognosticheskoe issledovanie nizinnyh drevesno-travjanyh vidov torfa razlichnogo proishozhdenija dlja obosnovanija ih ispol'zovanija v kachestve perspektivnyh istochnikov biologicheski aktivnyh guminovyh kislot. Himija rastitel'nogo syr'ja. 2022; 1: 277–288.
  5. Avvakumova N.P., Mizina P.G., Krivopalova M.A., Zhdanova A.V., Katunina E.E., Glubokova M.N. Protivovospalitel'naja aktivnost' guminovyh kislot peloidov. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2018; 21(6): 33–37.
  6. Buzlama A.V., Starov V.A. Izuchenie pronicaemosti membran jeritrocitov pod vlijaniem sapropelevogo gumata. Zdorov'e i obrazovanie v XXI veke. 2010; 12(4): 441–442.
  7. Zhernov Ju.V., Haitov M.R. Mikrobicidy dlja topicheskoj immunoprofilaktiki VICh-infekcii. Bjulleten' sibirskoj mediciny. 2019; 18(1): 49–59.
  8. Zhernov Ju.V., Zhdanova A.V., Avvakumova N.P., Haitov M.R. Immunoantioksidantnaja aktivnost' guminovyh kislot. Rossijskij allergologicheskij zhurnal. 2018; 15(S1-2): 30–31.
  9. Zhernov Y.V., Konstantinov A.I., Zherebker A., Nikolaev E., Orlov A.A., Savinykh M. I., Kornilaeva G.V., Karamov E.V., Perminova I.V. Antiviral activity of natural humic substances and shilajit materials against HIV-1: relation to structure. Environ. Res. 2020: 110312; https://doi.org/10.1016/j.envres. 2020.110312.
  10. Joly H.A., Li H., Belzile N. Pyrolysis gas chromatography – mass spectrometry of humic substances extracted from Canadian lake sediments. Canadian Journal of Chemistry. 2000; 78(1): 51–63; https://doi.org/10.1139/v99-228.
  11. Zykova M.V., Brazovsky K.S., Veretennikova E.E., Danilets M.G., Logvinova L.A., Romanenko S.V., Trofimova E.S., Ligacheva A.A., Bratishko K.A., Yusubov M.S., Lyapkov A.A., Belousov M.V. New artificial network model to estimate biological activity of peat humic acids. Environ. Res. 2020; 191: 109999; https://doi.org/ 10.1016/j.envres.2020.109999.
  12. Avvakumova N.P., Stepanov G.V., Krivopalova M.A., Glubokova M.N., Katunina E.E., Zhdanova A.V., Ul'janova L.G. Sposob poluchenija guminovyh kislot iz nekondicionnyh peloidov. Patent na izobretenie 2744469 C1, 09.03.2021. Zajavka№ 2020131211 от 21.09.2020.
  13. Griffiths P., de Hasseth J.A. Fourier Transform Infrared Spectrometry (2nd ed.). Wiley-Blackwell. 2007: 75–76.
  14. argapolov A.V. Ispol'zovanie IK-spektroskopii v medicine, jekologii i farmacii. Pod red. prof. A.V. Kargapolova. Tver': OOO «Izdatel'stvo «Triada», 2003. С. 216.