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BIOCOLLECTION OF PHARMACEUTICAL PARASITIC STRAINS OF CLAVICEPS PURPUREA – BASE FOR SELECTION OF NEW LINES PRODUCING ERGOALKALOIDS IN VITRO

DOI: https://doi.org/10.29296/25877313-2023-08-03
Issue: 
8
Year: 
2023

A.A. Volnin
Ph.D. (Biol.), Leading Research Scientist, Biotechnology Laboratory,
FSBSI All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)
E-mail: volnin.a@mail.ru
N.S. Tsybulko
Ph.D. (Pharm.), Engineer, Biotechnology Laboratory,
FSBSI All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)
P.S. Savin
Ph.D. (Biol.), Leading Research Scientist, Biotechnology Laboratory,
FSBSI All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)
S.B. Myasnikova
Research Scientist, Biotechnology Laboratory,
FSBSI All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow, Russia)
R.I. Bobyleva

Relevance. Ergot Claviceps purpurea is a very important source of pharmaceutical raw materials (ergoalkaloids). Aim of the study – for biocollection maintenance two parasitic strains of Claviceps purpurea (Fries) Tulasne were reproduced by in planta cultivation on winter rye: ergotoxin A-6, ergotoxin VKM-F-2450-D. Material and methods. Spectrophotometric determination of total content of indole alkaloids (with the van Urk reagent) and qualitative-quantitative selective determination of the alkaloid composition by thin-layer chromatography (TLC) with densitometric scanning of chromatograms were used. Results. Maternal sclerotia contained from 0.6 to 1 g of alkaloids per 100 g of sample for VKM-F-2450-D, from 0.5 to 0.7 g per 100 g of sample for A-6. VKM-F-2450-D and A-6 contained from 30% to 40% of the sum of α-ergocryptine and ergocornine and approximately 20% of β-ergocryptine. New gen-eration sclerotia contained from 0.31 to 0.76 g of alkaloids per 100 g of sample for VKM-F-2450-D, from 0.21 to 0.62 g per 100 g of sample for A-6. VKM-F-2450-D and A-6 contained from 40% to 62% and from 21% to 66% of the sum of α-ergocryptine and ergocornine, from 13% to 22% and from 13% to 36% of β-ergocryptine respectively. Conclusion. Two stable lines (ergotoxin A-6-S, ergotoxin ВKM-F-2450-D-S) with in vitro biosynthesis of ergoalkaloids with purple pigmentations features were detected, isolated and subcultivated by morphological selection in axenic culture. Accumulation and retention of Claviceps genetic resources is very actual: biocollection of parasitic strains is a good reserve for selection of new saprophytic lines.
Keywords: 
biocollection
ergot
ergoalkaloids
parasitic strains
Claviceps purpurea
axenic culture
mycelia.
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