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DEVELOPMENT OF QUALITY INDICATORS OF A NEW BIOSTIMULANT FROM PARACETAMOL WASTE

DOI: https://doi.org/10.29296/25877313-2023-10-01
Issue: 
10
Year: 
2023

E.V. Vikhareva
Dr.Sc. (Pharm.), Professor, Head of Department of Analytical Chemistry,
Perm State Pharmaceutical Academy (Perm, Russia)
E-mail: ajm@perm.ru;
https://orcid.org/0000-0002-7202-0073
E.D. Gapechkina
Post-graduate Student, Department of Analytical Chemistry,
Perm State Pharmaceutical Academy (Perm, Russia)
E-mail: elizaveta.gapechkina@gmail.com;
https://orcid.org/0000-0002-4626-3143
I.I. Mishenina
Ph.D. (Pharm.), Associate Professor, Department of Pharmaceutical Technology,
Perm State Pharmaceutical Academy (Perm, Russia)
E-mail: irin-mishenin@yandex.ru;
https://orcid.org/0000-0002-6496-7427
E.N. Lyust
Ph.D. (Pharm.), Associate Professor, Department of toxicological chemistry,
Perm State Pharmaceutical Academy (Perm, Russia)
E-mail: elenalyust@mail.ru;
https://orcid.org/0000-0002-1943-2669
M.I. Richkova
Ph.D. (Biol.), Leading Engineer,
“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” –
a branch of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences (Perm, Russia)
E-mail: richkova@iegm.ru; https://orcid.org/0000-0002-6598-5870

Introduction. In recent years, there has been a substantial rise in the utilization of biostimulants in agricultural crop production. The authors’ exten-sive research over the years has revealed that the byproduct of bacterial degradation of paracetamol exhibits a notable stimulatory effect on various medicinal and agricultural plants from different families. It serves as an inducer for the accumulation of biologically active substances in these plants, thereby making it a promising and efficient biostimulant. The purpose of this study is to establish a comprehensive set of indicators and create a preliminary specification for the quality control of the bacte-rial degradation product of paracetamol, which serves as a new biostimulant for medicinal and agricultural plants. Material and methods. For this study, we utilized the bacterial degradation product of paracetamol, derived from a pharmaceutical substance with an expired shelf life. The product was obtained from the Laboratory of Alkanotrophic Microorganisms, Perm Federal Research Center UB RAS (Perm). We employed various methods to analyze the product, including determining the melting point through the capillary method, assessing lead, cadmium, and arsenic content using an atomic absorption spectrometer with electrothermal atomization (MGA-915M, Lumex, Russia) and the Mga software on a personal computer running the Windows®-2000/XP operating system. Additionally, we recorded the IR spectrum using a Specord M80 IR spectrometer (Carl Zeiss Industrielle Messtechnik GmbH, Germany), and the electronic spectra were obtained with a Lambda EZ 201 spectrophotometer (Perki-nElmer, USA). The quantification of phenolic hydroxyl and carboxyl groups was performed through back acid-base potentiometric titration. Results: The product of bacterial degradation of paracetamol is practically insoluble in water, ether, very slightly soluble in chloroform, slightly soluble in hexane and acetonitrile, soluble in acetone and ethyl acetate, freely soluble in methyl alcohol, 95% ethyl alcohol and dimethyl sulfoxide. The melting point ranged from 150 to 206 °C, with decomposition. The heavy metal and arsenic content in samples of the product with varying shelf life complied with the standards outlined in general pharmacopoeia monograph 1.5.3.0009.15. The IR spectrum exhibited characteristic absorption bands of func-tional groups, including C–H aromatic (3042 cm1), –OH phenolic (3425 cm1), –NH– (3200 cm1), and C= N (1609 cm1). The electronic spectra of product solutions, in the concentration range of 5·106 to 2.5·105 g/cm3, displayed absorption maxima at λ=218 and λ=318 nm. The content of phenolic hydroxyls in the product composition ranged from 4.22 to 5.16 mmol-eq/g, while carboxyl groups ranged from 1.76 to 3.05 mmol-eq/g, comparable to the quantities found in humic acids. Conclusions. We have established a comprehensive list of indicators and developed a preliminary specification for the quality control of the bacterial degradation product of paracetamol as a biostimulant for medicinal and agricultural plants.

Keywords: 
product of bacterial degradation of paracetamol
biostimulant
quality indicators
specification.

References: 
  1. Tripathi A., Pandey P., Tripathi S.N., Kalra A. Perspectives and potential applications of endophytic microorganisms in cultivation of medicinal and aromatic plants. Front Plant Sci. 2022; 13: 985429. DOI: 10.3389/fpls.2022.985429.
  2. Evropejskij sovet industrii biostimuljatorov. Dostupno po: www.biostimulants.eu. Дата обращения: 03.07.2023.
  3. Kumari M., Swarupa P., Kesari K.K., Kumar A. Microbial Inoculants as Plant Biostimulants: A Review on Risk Status. Life (Basel). 2022; 13(1): 12. DOI: 10.3390/life13010012.
  4. Jahin O.I., Lubjanov A.A., Jahin I.A. Biostimuljatory v agrotehnologijah: problemy, reshenija, perspektivy. Agro-himicheskij vestnik. 2016; 1: 15–21.
  5. Mattedi A., Sabbi E., Farda B., Djebaili R., Mitra D., Ercole C., Cacchio P., Del Gallo M., Pellegrini M. Solid-State Fermentation: Applications and Future Perspectives for Biostimulant and Biopesticides Production. Microorganisms. 2023; 11(6): 1408. DOI: 10.3390/microorganisms11061408.
  6. Giordana A., Malandrino M., Zambon A., Lusvardi G., Operti L., Cerrato G. Biostimulants derived from organic urban wastes and biomasses: An innovative approach. Front Chem. 2023; 11: 969865. DOI: 10.3389/fchem.2023.969865.
  7. Llorens E., Agustí-Brisach C. Biocontrol of Plant Diseases by Means of Antagonist Microorganisms, Biostimulants and Induced Resistance as Alternatives to Chemicals. Plants (Basel). 2022; 11(24): 3521. DOI: 10.3390/plants11243521.
  8. Ma Y., Freitas H., Dias M.C. Strategies and prospects for biostimulants to alleviate abiotic stress in plants. Front Plant Sci. 2022; 13: 1024243. DOI: 10.3389/fpls.2022.1024243.
  9. Gupta S., Bhattacharyya P., Kulkarni M.G., Doležal K. Editorial: Growth regulators and biostimulants: upcoming opportunities. Front Plant Sci. 2023; 14: 1209499. DOI: 10.3389/fpls.2023.1209499.
  10. Gosudarstvennyj katalog pesticidov i agrohimikatov, razreshennyh k primeneniju na territorii Rossijskoj Federacii. Minsel'hoz Rossii. M. 05.06.2023. Dostupno po: https://mcx.gov.ru. Data obrashhenija: 03.07.2023.
  11. Korotaev M.Ju., Vihareva E.V., Ivshina I.B. Melani-zacija poliaminofenolov v processe biotransformacii paracetamola kletkami Rhodococcus ruber IJeGM 77. Vestnik Permskogo universiteta. Ser. Biologija. 2016; 2: 166–170.
  12. GOST 32419-2022 Klassifikacija opasnosti himicheskoj produkcii. Obshhie trebovanija. Utv. prik. Feder. agentstva po tehn. regulir. i metrol. ot 07.07.2022 g. № 572-st. Dostupno po: https://base.garant.ru/405090455. Data obrashhenija: 03.07.2023.
  13. Mishenina I.I., Vihareva E.V., Guljaev D.K. Issledo-vanie fitostimulirujushhego dejstvija produktov bio-destrukcii paracetamola na lekarstvennye rastenija semejstva Jasnotkovye. Mediko-farmacevticheskij zhurnal «Pul's». 2020; 22(4): 62–66. DOI: 10.26787/nydha-2686-6838-2020-22-4-62-66.
  14. Vihareva E.V., Slabinskaja E.A., Mishenina I.I., Rubcov D.F., Rychkova M.I. Stimulirujushhee dejstvie produkta biodestrukcii paracetamola na krapivu dvudomnuju. Voprosy biologicheskoj, medicinskoj i farmacevtiches-koj himii. 2023; 26(5): 32–37. DOI: 10.29296/25877313-2023-05-05.
  15. Vihareva E.V., Mishenina I.I., Agafonceva A.V., Rychkova M.I. Fitostimulirujushhaja aktivnost' produkta bio-destrukcii paracetamolsoderzhashhih othodov. Formuly Farmacii. 2022; 4(3): 10–17. DOI: 10.17816/phf119839.
  16. Vihareva E.V., Mishenina I.I., Gapechkina E.D., Seljaninov A.A., Rychkova M.I. Fitostimulirujushhee dejstvie produkta biodestrukcii paracetamola na kalendulu lekarstvennuju. Razrabotka i registracija lekarstvennyh sredstv. 2022; 11(4): 31–37. DOI: 10.33380/2305-2066-2022-11-4(1)-31-37.
  17. Metodicheskoe rukovodstvo po klassifikacii smesevyh agrohimikatov dlja celej gosudarstvennoj registracii v chasti ocenki biologicheskoj jeffektivnosti i reglamentov na territorii Rossijskoj Federacii. Minsel'hoz Rossii. Moskva, 2020. Dostupno po: https://mcx.gov.ru/upload/iblock/403/403690ae-ccc645e83d0655eaf6f51828.pdf. Data obrashhenija: 03.07.2023.
  18. Regional'naja specializirovannaja kollekcija alkano-trofnyh mikroorganizmov. Dostupno po: iegmcol.ru. Data obrashhenija: 03.07.2023.
  19. Federal'naja jelektronnaja medicinskaja biblioteka: ofic. sajt. M.: Gosudarstvennaja farmakopeja RF. XIV izd. 2018. T. I–IV. Dostupno po: https://femb.ru/re-cord/pharmacopea14. Data obrashhenija: 03.07.2023.
  20. Kovtun A.I., Hil'ko S.L., Rybachenko V.I. Potencio-metricheskoe titrovanie solej guminovyh kislot. Nauch-nye trudy Doneckogo nacional'nogo tehnicheskogo uni-versiteta. Serija: Himija i himicheskaja tehnologija. 2010; 15(163): 15–20.
  21. Ritchie J.D., Perdue E.M. Analytical constraints on acidic functional groups in humic substances. Organic Geochemistry. 2008; 39: 783–799.
  22. Korotaev M.Ju. Potenciometricheskoe opredelenie kislotnyh grupp v produktah bakterial'noj destrukcii paracetamola. Nauka i obrazovanie v zhizni sovremennogo obshhestva. Sb. nauchnyh trudov po materialam Mezhdunar. nauchno-prakticheskoj konferencii. 2015; 2: 87–89.