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Эко-Вектор (Россия)

IMMUNOMODULATING ACTIVITY OF INDIVIDUAL SUBSTANСES ISOLATED FROM THE AERIAL PART OF RHAPONTICUM UNIFLORUM (L.) DC IN EXPERIMENTAL IMMUNODEFICIENCY

DOI: https://doi.org/10.29296/25877313-2024-04-09
Issue: 
4
Year: 
2024

V.B. Khobrakova
Dr.Sc. (Biol.), Associate Professor, Head of the Laboratory of Experimental Pharmacology,
Institute of General and Experimental Biology SB RAS;
Professor of the Department of Human General Pathology,
Medical institute, Buryat State University named after DorzhiBanzarov (Ulan-Ude, Republic of Buryatia, Russia)
E-mail: val0808@mail.ru
Yu.A. Tugarina
Post-graduate Student, Laboratory of Experimental Pharmacology,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Republic of Buryatia, Russia)
E-mail: ytugarina@mail.ru
D.N. Olennikov
D.Sc (Pharm.), Head of the Laboratory of Biomedical Research,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Republic of Buryatia, Russia)
E-mail: olennikovdn@mail.ru
S.I. Pavlova
Dr.Sc. (Med.), Professor, Head of the Department of Pharmacology, Clinical Pharmacology and Biochemistry, Faculty of Medicine,
Chuvash State University named after I.N. Ulyanov of the Ministry of Science and Higher Education of the Russian Federation
(Cheboksary, Russia)
E-mail: flavonoid@yandex.ru

Introduction. The development and introduction of new drugs with immunomodulatory effects is one of the priorities of modern medicine. Numerous studies have proven the immunotropic activity of individual substances isolated from medicinal plants (flavonoids, phenylpropanoids, polysaccharides, ecdysteroids, terpenoids, etc.). In this study, it is of interest to determine the immunomodulatory effect of individual substances isolated from Rhaponticum uniflorum (L.) DC. The purpose of the study. Determine the immunomodulatory properties of 20-hydroxyecdysone, 5-О-caffeoylquinic acid and arabino-3,6-galactan, isolated from the aerial part of Rhaponticum uniflorum (L.) DC, in relation to the cellular and humoral components of the immune response during exper-imental immunosuppression caused by cyclophosphamide. Material and methods. The experiments were performed on 100 male mice of the F1 line (CBAxC57Bl/6) weighing 18-20 g. Immunodeficiency was simulated by in traperitoneal administration of cyclophosphamide at a dose of 250 mg/kg once. The experimental group of animals received individual substances intragastricallyin the following doses: 5-О-caffeoylquinic acid - 10 mg/kg, arabino-3,6-galactan - 10 mg/kg, 20-hydroxyecdysone - 3 mg/kg, once a day for 14 days. The intact group of mice received purified water according to the analogous scheme. The state of humoral chain of the im-mune response was estimated according to the quantity of antibody-forming cells (AFC) determined with the use of Cunningham's plaque assay (1965). The state of the cellular chain of the immune response was estimated according to the delayed hypersensitivity reaction. Study results. It has been established that 20-hydroxyecdysone, 5-О-caffeoylquinic acid and arabino-3,6-galactan, isolated from the aerial part of Rhaponticum uniflorum (L.) DC, have immunomodulatory effect on the cellular and humoral components of the immune response in immunosuppres-sion caused by cyclophosphamide. Conclusions. The obtained results argues the feasibility of further research of individual substances, isolated from the aerial part of Rhaponticum uni-florum (L.) DC, with a view to creating new effective immunomodulators of plant origin.
Keywords: 
dry extract from Rhaponticum uniflorum (L.) DC
20-hydroxyecdysone
5-О-caffeylquinic acid
arabino-3
6-galactan
immunomodulatory activity
cyclophos-phamide
immunosuppression
delayed-type hypersensitivity reaction
antibody genesis.
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