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DEVELOPMENT AND VALIDATION OF METHODS FOR THE SPECTROPHOTOMETRIC DETERMINATION OF PROSPIDIUM CHLORIDE FOR IDENTIFICATION AND ASSAY ANALYSIS
DOI: https://doi.org/10.29296/25877313-2024-06-02
Issue:
6
Year:
2024
Objective. The work is devoted to the development and validation of UV-spectrophotometric analysis of propidium chloride, an antitumour drug of the dyspyropiperazine group. The relevance of the study is due to the need to introduce into the practice of quality control of prospidium chloride analysis method characterised by high sensitivity, accuracy, rapidity and availability.
Material and methods. The object of the study was a medicinal substance-powder Prospidin (Prospidium chloride, series 271222, expiry date until 12.2027g, produced by «Unitehprom BSU», Minsk). Solutions with working concentration range from 0.05 mol/l to 0.2 mol/l for spectrophotometric analysis were prepared using purified water. Absorption spectra with determination of analytical wavelength (λmax, nm) and value of molar absorption coefficient ε, (l mol-1cm-1) in the range from 200 nm to 400 nm were obtained and analysed on Agilent Cary 60 equipment. Validation parameters were determined in accordance with the regulations of the General Pharmacopoeial Article 1.1.0012.15.
Results. Validation evaluation of the developed analytical techniques for the determination of authenticity and active ingredient content by UV spectro-photometry included tests for specificity (in the presence of NaCl 0.9% solution), analytical range (0.05−0.2 mol/L), linearity (r=0.9997), accuracy, preci-sion in terms of repeatability 3.89±0.24 ( ±SD) and intra-laboratory reproducibility (ε=2.1%) and stability.
Conclusion. The analysis of electronic spectra of aqueous solutions of propidium chloride demonstrated the presence of R-band absorption (German radikalartig − radical, for systems with unshared electron pairs) with a characteristic wavelength in the far-UV region at λmax=282±0.4 nm, and λmin=255±0.4 nm. It was found that the absorption at λmax in the concentration range from 0.05 mol/L to 0.2 mol/L of a solution of propidine chloride containing nitrogen heteroatoms with unshared electron pairs is due to the implementation of the so-called formally forbidden n→σ* electronic transition involving molecular orbitals. The characteristic appearance of the electronic spectrum with λmax and λmin, compliance with the Beer–Lambert law in the given concentration range, the calculated value of the molar extinction coefficient ε (l mol1cm1) − allow to use the above described spectral characteristics to determine the authenticity and content of the active substance in quality control of prospidium chloride.
Keywords:
propidium chloride
electronic absorption spectrum
molecular orbitals
electronic transitions
molar extinction coefficient
qualitative and quantitative analysis.
References:
- Кладиев А.А., Степанова Е.В., Соломко Э.Ш. и др. Антиан-гиогенные свойства проспидина in vitro. Российский биотера-певтический журнал. 2009; 3(8): 45−50. [Kladiev AA, Stepanova EV, Solomko E.Sh. et al. Anti-angiogenic properties of propidin in vitro. Russian Biotherapeutic Journal. 2009; 3(8): 45−50. (In Russ.)].
- Евстигнеева Р.П. Тонкий органический синтез. Учеб. пособие для вузов; М.: Химия; 1991; 184 с. [Evstigneeva R.P. Fine organic synthesis. Textbook for universities; M.: Chemistry 2009; 3(8): 45−50. (In Russ.)].
- Pai V.B., Nahata M.C. Cardiotoxicity of chemotherapeutic agents: incidence, treatment and prevention. Drug Saf. 2000; 22(4): 263−302.
- Zhang L., Ren W., Wang X. at al. Discovery of novel polycyclic spiro-fused carbocyclicoxindole-based anticancer agents. Eur J Med Chem. 2017; 27(126): 1071−1082.
- Nilapwar S.M., Nardelli M., Westerhoff H.V., Verma M. Absorption spectroscopy. Methods Enzymol. 2011; 500: 59−75.
- Röder A., Skov A.B., Boguslavskiy A.E. et al. VUV excited-state dynamics of cyclic ethers as a function of ring size. Phys Chem Chem Phys. 2020; 22(45): 26241−26254.
- Государственная фармакопея Российской Федерации. Изд. XV. Т. 1. Общие положения. Общая фармакопейная статья ОФС.1.1.0012 [Электронный ресурс]. Режим доступа: https://pharmacopoeia.regmed.ru/pharmacopoeia/iz-danie-15/1/1-1/validatsiya-analiticheskikh-metodik/. [State Pharmacopoeia of the Russian Federation. XV. V. 1. General provisions. General pharmacopoeia article OFS.1.1.0012 [Electronic resource]. Access: https://pharmacopoeia.reg-med.ru/pharmacopoeia/izdanie-15/1/1-1/validatsiya-analiti-cheskikh-metodik/. (In Russ.)].
- Государственная фармакопея Российской Федерации. Изд. XV. Т. 1. Общие положения. Общая фармакопейная статья ОФС.1.1.0013 [Электронный ресурс]. Режим доступа: https://pharmacopoeia.regmed.ru/pharmaco-poeia/iz-danie-15/1/1-1/statisticheskaya-obrabotka-rezultatov-fizi-cheskikh-fiziko-khimicheskikh-i-khimicheskikh-ispytaniy/. [State Pharmacopoeia of the Russian Federation. XV. V. 1. General provisions. General pharmacopoeia article OFS.1.1.0013 [Electronic resource]. Access: https://phar-macopoeia.regmed.ru/pharmacopoeia/izdanie-15/1/1-1/statis-ticheskaya-obrabotka-rezultatov-fizicheskikh-fiziko-khimi-cheskikh-i-khimicheskikh-ispytaniy/. (In Russ.)].