Нажмите на эту строку чтобы перейти к Новостям сайта "Русский врач"

Перейти
на сайт
журнала
"Врач"
Перейти на сайт журнала "Медицинская сестра"
Перейти на сайт журнала "Фармация"
Перейти на сайт журнала "Молекулярная медицина"
Перейти на сайт журнала "Вопросы биологической, медицинской и фармацевтической химии"
Журнал включен в российские и международные библиотечные и реферативные базы данных

ВАК (Россия)
РИНЦ (Россия)
Эко-Вектор (Россия)

SESELI LIBANOTIS (L.) W.D.J. KOCH (APIACEAE): CHEMICAL COMPOSITION OF ESSENTIAL OILS, THEIR ANTIBACTERIAL AND CYTOTOXIC ACTIVITIES

DOI: https://doi.org/10.29296/25877313-2024-06-04
Issue: 
6
Year: 
2024

N.P. Rabdanova(1)
Junior Research Scientist, Laboratory of Physiologically Active Substances and Phyto-engeneering
E-mail: nrabdanova@list.ru
A.P. Tsybdenova(2,3)
Ph.D. (Biol.), Senior Lecturer; Head of Small Innovative Enterprise «Baikal Center of Biotechnologies»
V.V. Taraskin(1,4)
Ph.D. (Pharm.), Head of Laboratory; Senior Research Scientist, Laboratory of Medicinal Herbs
Zh.A. Tykheev(1,4)
Ph.D. (Pharm.), Head of Laboratory of Physiologically Active Substances and Phyto-engeneering;
Senior Research Scientist, Laboratory of Medicinal Herbs
B.M. Zhigmitsyrenova(1)
Ph.D. (Pharm.), Senior Research Scientis, Laboratory of Physiologically Active Substances and Phyto-engeneering
D.A. Dargeev(5)
Biologist, Bacteriological Laboratory
S.S. Babinov(5)
Laboratory Medical Technician, Bacteriological Laboratory
1 -Baikal Institute of Nature Management Siberian branch of the Russian Academy of sciences, Sakhyanovoy str. 6, Ulan-Ude, 670047, Republic of Buryatia, Russian Federation
2 -Banzarov Buryat State University, Smolin str. 24a, Ulan-Ude, 670000, Republic of Buryatia, Russian Federation
3 LLC Small Innovative Enterprise «Baikal Center of Biotechnologies», Khakhalova str. 12а, Ulan-Ude, 670034, Republic of Buryatia, Russian Federation
4 -V.R. Filipov Buryat State Agricultural Academy, Pushkina str. 8, Ulan-Ude, 670010, Republic of Buryatia, Russian Federation
5 -State Autonomous Health Care Institution «Republican Clinical Hospital of Emergency Medical Care named after A A. Angapov», Profsoyuznaya str. 48а, Ulan-Ude, 670000, Republic of Buryatia, Russian Federation

Introduction. Infectious diseases and cancer remain among the leading causes of death worldwide. In this regard, the search for biologically active molecules from among synthetic and natural compounds for the purpose of creating medicines remains an urgent task of modern science. Plants that have impressive natural and biotechnological resource potential, taking into account modern advances in the field of ex situ, in situ and in vitro cultiva-tion, remain a promising source of native substances. One of the promising sources of biologically active substances is a perennial plant, the Seseli libanotis (L.) W.D.J. Koch, Apiaceae, growing in central, eastern Europe and Asia in dry pastures and forest edges. The main components of the genus Seseli L. are furo- and pyranocoumarins, essential oils and polyacetylene compounds, which exhibit antibacterial, antifungal, insecticidal, diuretic, antiviral, anti-inflammatory, and antitumor properties. The aim – study of the chemical composition, antibacterial and cytotoxic activities of S. libanotis roots essential oil. Material and methods. The roots were collected in places of natural growth in September 2022, during the fruiting period (Irkutsk region, Maloye Goloustnoye village). The voucher specimen is stored in the herbarium of the Institute of Ecological Ecology SB RAS (UUH 020438). Obtaining essential oils. Essential oils were obtained by hydrodistillation. The qualitative composition and quantitative content of essential oil compo-nents were studied by GC/MS. The study of the antibacterial activity of essential oils was carried out in vitro on test cultures of gram-positive and gram-negative bacteria (Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomona saeruginosa, Salmonella enterica) using the disc diffusion method. Cell viability assay was performed on HeLa cell lines using trypan blue. Results. The composition and quantitative content of the components of essential oils of the roots of S. libanotis of the Baikal flora were studied for the first time. 28 components of predominantly monoterpene nature have been identified, the main ones being limonene (57,60%), α-pinene (6,88%), cis-β-ocimene (6,75%). Also found were fatty acids, polyacetylene-falcorinol and 7-isopen-tinyloxycoumarin. Essential oils of S libanotis have antibacterial activity against gram-positive bacteria Staphylococcus aureus, Streptococcus pyogenes and gram-negative bacteria Escherichia coli. Cultivation of Hela with S. libanotis essential oils resulted in decreased viability in the concentration range of 0.04–2.56 μL/mL for 72 hours. Conclusions. Essential oils of roots of S. libanotis, with high yield, containing falcarinol and coumarin, have antibacterial and cytotoxic activity.

Keywords: 
Seseli libanotis
roots of Seseli libanotis
essential oils
antibacterial and cytotoxic activities.

References: 
  1. Newman D.J., Cragg G.M. Natural products as sources of new drugs over the nearly four decades from. Journal of Natural Products. 2020; 83(3): 770–803.
  2. Reduron J.P. Taxonomy, origin and importance of the Apiaceae family. In Carrots and related Apiaceae crops; Wallingford, Oxfordshire, UK: CABI Publishing. 2020; 1–8.
  3. Onder A., Nahar L., Cinar A.S. et al. The genus Seseli L.: A comprehensive review on traditional uses, phytochemistry, and pharmacological properties. Journal of Herbal Medicine. 2023; 38: 2210–8033.
  4. Pimenov M.G., Ostroumova T.A. Zontichnye (Umbelliferae) Rossii. M.: Tovarishhestvo nauchnyh izdanij KMK. 2012; 477 s.
  5. Anenhonov O.A., Pyhalova T.D., Osipov K.I. i dr. Opredelitel' rastenij Burjatii. Ulan-Udje: OAO «Respublikanskaja tipografija». 2001; 672 s.
  6. Belenovskaja L.M., Bitjukova N.V., Bobyleva N.S. i dr. Rastitel'nye resursy Rossii. Dikorastushhie cvetkovye rastenija, ih komponentnyj sostav i biologicheskaja ak-tivnost'. Semejstva Fabaceae–Apiaceae. SPb.: Tovarishhestvo nauchnyh izdanij KMK. 2010; 601 s.
  7. Tkachev A.V. Issledovanie letuchih veshhestv rastenij. Novosibirsk: izdatel'sko-poligraficheskoe predprijatie «Ofset». 2008; 969 s.
  8. Murray P.R., Baron E.J., Jorgensen J.H. et al. Manual of Clinical Microbiology. Washington: ASM Press. 2007; 2256.
  9. Mumivand H., Aghemiri A., Aghemiri A. et al. Ferulago angulata and Tetrataenium lasiopetalum: Essential oils composition and antibacterial activity of the oils and extracts. Biocatalysis and Agricultural Biotechnology. 2019; 22: 101407.
  10. Gonc M.J., Tavares A.C., Cavaleiroa C. et al. Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops and Products. 2012; 39: 204–209.
  11. Hramchenkova O.M., Matveenkov M.V. Citotoksicheskaja aktivnost' jekstraktov iz chetyreh vidov lishajnikov v ot-noshenii kul'tur opuholevyh kletok. Zhurnal Belo-russkogo gosudarstvennogo universiteta. Jekologija. 2018; 2: 88–95.
  12. Preziuso F., Genovese S., Marchetti L. et al. 7-Isopentenyloxycoumarin: What Is New across the Last Decade. Molecules. 2020; 25 (24): 5923.
  13. Rosa A., Nieddu, M., Petretto G.L. et al. Chemical composition and in vitro bioactivity of essential oil obtained from the flavedo of "Pompia", an ancient Sardinian fruit. Journal of Essential Oil Research. 2019; 31: 390–399.
  14. ChunYan H., Hong P., ZhenYu Z. et al. Evaluation of antioxidant and antitumour activities of lemon essential oil. Journal of Medicinal Plants Research. 2010; 4: 1910–1915.