ЕVALUATION OF SUM OF FLAVONOIDS, TANNINS AND POLYSACCHARIDES IN SAPOSHNIKOVIA DIVARICATA RADICES

DOI: https://doi.org/10.29296/25877313-2019-10-04
Issue: 
10
Year: 
2019

B.M. Urbagarova Post-graduate Student, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management Siberian branch of the Russian Academy of Sciences; Lecturer, Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude) E-mail: urbagarova.bayarma@mail.ru V.V. Taraskin Ph.D. (Pharm.), Senior Research Scientist, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management of the Siberian branch of the Russian Academy of Sciences; Senior Lecturer, Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude)

The aim of this work was to determine the total sum of flavonoids, tannins and the quantitative content of polysaccharide fractions of Saposhnikoviae divaricatae radices collected in Russia, Mongolia and obtained in China. Plant material on different phases of development was collected during field works in Tarbagataisky, Mukhorshibirsky, Ivolginsky and Kyakhtinsky districts of the Republic of Buryatia (Russia), in Aginsky district of the Transbaikal Territory (Russia) and in Khentei aimak (Mongolia), and obtained in a pharmacy store in Xining, Qinghai Province (China). The total sum of flavonoids was determined by spectrophotometric method. The content of tannins was determined by permanganatometric method in terms of tannin. The quantitative content of polysaccharide fractions was carried out by gravimetric method. The total sum of flavonoids in terms of rutin in Saposhnikoviae divaricatae radices was 0.290.48%. The quantitative content of tannins was ranged from 0.38 to 0.55%. Water-soluble polysaccharides were represented by an amorphous yellowish-gray powder, soluble in water. The content ranged from 6.8 to 8.3%. The fraction of pectin substances was represented by an amorphous gray powder, soluble in water. The quantitative content of the sample from Tarbagataisky region was the highest  1.1%, while the amount of the rest ranged from 0.3 to 0.4%. The quantitative content of hemicellulose A in studied samples varied significantly, and ranged from 2.5% to 13.8%. The yield of grayish-brown powder of hemicellulose B was 1.8-14.3%. Conclusion. For the first time the content of the total sum of flavonoids, tannins and polysaccharides in the roots of Saposhnikovia divaricata from Russian and Mongolian flora was studied in this research. It was found that this species accumulates significant quantity of flavonoids and tannins along with coumarins and chromones. Relatively high content of water-soluble polysaccharides in S. divaricata shows promising studying of their composition and structure.

Keywords: 
Saposhnikovia divaricata (Turcz.) Schischkin.
flavonoids
tannins
polysaccharides

References: 
  1. Yang J-M. Jiang H., Dai H-L., Wang Z.-W., Jia G.-Z., Meng X.-C. Feeble Antipyretic, Analgesic, and Anti-inflammatory Activities were Found with Regular Dose 4’-O-β-D-Glucosyl-5-OMethylvisamminol, One of the Conventional Marker Compounds for Quality Evaluation of Radix Saposhnikoviae // Pharmacognosy Magazine. 2017; 13(49): 168-74.
  2. Heuberger H., Bauer R., Friedl F., Heubl G., Hummelsberger J., Nögel R., Seidenberger R., Torres-Londoño P. Cultivation and Breeding of Chinese Medicinal Plants in Germany // Planta Med. 2010; 76: 1956–62.
  3. Jin G., Li J., Piao H. Chemical constituents of Ledebouriella seseloides Wolff // ZhongguoZhong Yao ZaZhi. 1992; 64: 38–40.
  4. Han B., Dai Y., Wu H., Zhang Yu. Cimifugin Inhibits Inflammatory Responses of RAW264.7 Cells Induced by Lipopolysaccharide // Medical Science Monitor. 2019; .25: 409–417.
  5. Jiang H., Yang J.M., Jia G.Z., Dai H-L., Cao L., Meng X.-C. Phys-ical and Ecological Impacts of Chromones of Fresh Root of Saposhnikovia divaricata Exposure to High Temperature // Russian Journal of Plant Physiology. 2018; 65(5): 680–87.
  6. Tai J., Cheung S, Tai J. Anti-proliferative and antioxidant activities of Saposhnikovia divaricata // Oncology reports. 2007; 18: 227–34.
  7. Kim M., Seo K.-S., Yun K.W. Antimicrobial and Antioxidant Activi-ty of Saposhnikovia divaricata, Peucedanum japonicum and Glehnia littoralis // Indian Journal of Pharmaceutical Sciences. 2018; 80(3): 560–565.
  8. Yang J.-M., Jiang H., Dai H.-L., Wang Z.W., Jia G.Z., Meng X.C. Polysaccharide enhances Radix Saposhnikoviae efficacy through inhibiting chromones decomposition in intestinal tract // Scientific Reports. 2016; 6: 1–8.
  9. Martynov A.M., Dargaeva T.D. Sostav polisaharidnyh kompleksov Viola langsdorfii // Sibirskij meditsinskij zhurnal. 2010; 2: 114–116 (Martynov A.M., Dargaeva T.D. Sostav polisaharidnyh kompleksov Viola langsdorfii // Sibirskij medicin-skij zhurnal. 2010; 2: 114–116).