SPECIFIC FEATURES OF SERUM AMINO ACID CONCENTRATION IN PRESCHOOL CHILDREN WITH AUTISM SPECTRUM DISORDER

DOI: https://doi.org/10.29296/25877313-2020-02-04
Issue: 
2
Year: 
2020

A.V. Skalny Dr.Sc. (Med.), Professor, Head of Laboratory, Yaroslavl State University; Head of Laboratory, Sechenov University (Moscow); Head of Department, RUDN University (Moscow) T.V. Korobeynikova Ph.D. (Eng.), Department of Medical Elementology of the Medical Institute, Peoples' Friendship University of Russia; ANO “Center for Biotic Medicine” (Moscow) А.А. Skalny Assistant, Department of Medical Elementology of the Medical Institute, Peoples' Friendship University of Russia; ANO “Center for Biotic Medicine” (Moscow) Yu.N. Lobanova Ph.D. (Biol.), Senior Lecturer, Department of Medical Elementology of the Medical Institute, Peoples' Friendship University of Russia; ANO “Center for Biotic Medicine” (Moscow) M.G. Skalnaya Dr.Sc. (Med.), Professor, Peoples’ Friendship University of Russia; ANO “Center for Biotic Medicine” (Moscow) A.A. Tinkov Ph.D. (Med.), Research Scientist, Yaroslavl State University; Leader Research Scientist, Sechenov University; Peoples’ Friendship University of Russia (Moscow) E-mail: tinkov.a.a@gmail.com

The aim of this study was to investigate the serum concentration of amino acids, as well as their derivatives, in children with an autism spectrum disorder of preschool age. Material and methods. There are 35 boys with ASD (ICD-10: F84.0) of preschool age (3-6 years) and 35 healthy peers were examined. Determina-tion of serum concentration of amino acids and their derivatives was carried out by high performance liquid chromatography (HPLC) on a PerkinElmer S200 chromatograph (PerkinElmer, USA). Results. It was found that the concentration of leucine, lysine, threonine, tryptophan, arginine, glutamine, serine, 1-methylhistidine, α-aminobutyric ac-id and taurine was characterized by a significant decrease relative to the control values by 11%, 22%, 19%, 22%, 34%, 17%, 13%, 29%, 20% and 14%, respectively. It is also worth noting a 16% decrease in serum phenylalanine, approaching significant in significance. At the same time, the hy-droxyproline serum concentration in children with autism exceeded the corresponding parameters in the control group by more than 3 times. In ac-cordance with the results of a group comparison, a tight relationship with ASD was found for the concentration of hydroxyproline (β = 0.447; p = 0.001) and arginine (β = 0.238; p = 0.068). A model including the level of amino acids characterized by any significant group differences (1mh, Aab, Arg, Gln, Hypro, Leu, Lys, Ser, Thr, Trp, Tau) was associated with 26% of the variability of the presence of ASD (p = 0.001). Conclusions. Thus, the data obtained indicate a pronounced trend towards amino acid deficiency in children with ASD amid an increase in the concen-tration of hydroxyproline, which may be the link between autism and joint hypermobility. In turn, a deficiency of a number of amino acids, which are precursors of neurotransmitters, can lead to aggravation of neuropsychiatric disorders. In this regard, the correction of the amino acids metabolism may be one of the potential directions in the treatment of ASD

Keywords: 
amino acids
blood serum
children
ASD
HPLC

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