DEVELOPMENT OF APPROACHES TO STANDARDIZATION OF FRESH FRUITS OF CRATAEGUS SUBMOLLIS SARG

DOI: https://doi.org/10.29296/25877313-2020-03-07
Issue: 
3
Year: 
2020

V.A. Kurkin Dr.Sc. (Pharm.), Professor, Head of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Samara State Medical University E-mail: Kurkinvladimir@yandex.ru I.Kh. Shaikhutdinov Postgraduate Student, Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Samara State Medical University O.E. Pravdivtseva Dr.Sc. (Pharm.), Associate Professor, Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Samara State Medical University E.V. Avdeeva Dr.Sc. (Pharm.), Professor, Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Samara State Medical University A.V. Kurkina Dr.Sc. (Pharm.), Associate Professor, Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Samara State Medical University V.V. Stenyaeva Ph.D. (Pharm.), Associate Professor, Department of Pharmacognosy with Botany and the Bases of Phytotherapy, Samara State Medical University N.R. Varina Ph.D. (Pharm.), Associate Professor, Department of Pharmacognosy with Botany and the Bases of Phytotherapy, Samara State Medical University A.V. Zhdanovа Ph.D. (Pharm.), Associate Professor, Department of General, Bioinorganic and Bioorganic chemistry, Samara State Medical University

Actuality. The fruits of Crataegus submollis Sarg. (family Rosaceae) are a popular food product, but are not currently used in medical practice. Previ-ously, the possibility of using of the fresh fruits of Crataegus submollis Sarg. as a source of antidepressant and diuretic drugs has been shown. One of the new drugs may be a juice based on fresh fruits of Crataegus submollis Sarg. In еру fresh fruits of the Crataegus submollis Sarg. predominate the re-duced forms of flavonoids, namely procyanidins. An earlier analysis of the juice from fresh fruits of Crataegus submollis Sarg. with the using of spectrophotometry showed that the maximum absorption in the UV spectrum is 282 nm, which coincides with the maximum absorption of the solu-tion of catechin, a biogenetic precursor of procyanidins. The aim is the development of the method for quantitative determination of the total procyanidins in of the fresh fruits of Crataegus submollis Sarg. Material and methods. The object of the study was fresh fruits of Crataegus submollis Sarg. harvested in September 2019 on the territory of Samara and Samara region. The method of analysis was direct spectrophotometry at a wavelength of 282 nm calculated on catechin. Results. As a results of the study the method of quantitative determination of the total procyanidins (calculated on catechin) in fresh fruits of Cratae-gus submollis Sarg. by direct spectrophotometry at an analytical wavelength of 282 nm was developed. It was determined that the content of the total procyanidins in fresh fruits of Crataegus submollis Sarg. varies from 0,52±0,03% to 0,97±0,06% (calculated on catechin). The error of a single determi-nation with a 95% confidence probability is ±5,81 %. Conclusion. The highest content of procyanidins can be noted in the sepals and the skin of еру fresh fruits of Crataegus submollis Sarg. The fresh fruits of Crataegus submollis Sarg. are promising medicinal plant raw materials.

Keywords: 
Crataegus submollis Sarg.
fruits
flavonoids
procyanidins
catechin
spectrophotometry

References: 
  1. Derev'ya i kustarniki SSSR. T. 3. Izdanie Akademii nauk SSSR MoskvaLeningrad. 1954. 872 s.
  2. Enikeeva K.E., Hasanova S.R., Kudashkina N.V., Andersova P.A., Asadullina D.D., Yarochkina A.R. Vybor optimal'nyh uslovij izvlecheniya antocianovyh soedinenij iz plodov boyaryshnika myagkovatogo. Vestnik Bashkirskogo gosudarstvennogo medicinskogo universiteta (setevoe izdanie). 2018; 4:130133.
  3. Morozova T.V., Kurkin V.A., Pravdivceva O.E., Rozno S.A., Zhavkina T.M. Sravnitel'noe fitohimicheskoe issle¬dovanie plodov, pobegov i cvetkov nekotoryh vidov roda boyaryshnik. Aspirantskij vestnik Povolzh'ya. 2018; 12:2224.
  4. Gosudarstvennaya Farmakopeya Rossijskoj Federacii. Izd. 14-e. M.: Ministerstvo zdravoohraneniya RF. 2018. URL: http://www.femb.ru/femb/pharmacopea.php.
  5. Kurkina A.V. Flavonoidy farmakopejnyh rastenij: monografiya. Samara: OOO «Ofort», GBOU VPO SamGMU Minzdravsocrazvitiya Rossii. 2012. 290 s.
  6. Patent № 2698325 (RF). Sok iz svezhih plodov boyaryshnika myagkovatogo, obladayushchego diureticheskoj aktivnost'yu. 26.08.2019.
  7. Hishova O.M., Buzuk G.N. Kolichestvennoe opredelenie procianidinov plodov boyaryshnika. Himiko-farmacevticheskij zhurnal. 2006; 2:2022.
  8. European Pharmacopoeia. 8rd Edition. Nördlingen, 2013; 1: 1456 p.