PECULIARITIES OF REPRODUCTION OF POTENTILLA ALBA L. AND POTENTILLA MEGALANTHA L. IN VITRO

DOI: https://doi.org/10.29296/25877313-2020-03-09
Issue: 
3
Year: 
2020

N. A. Polyakov Ph.D. (Biol.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: polakov@yandex.ru E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Department of Biotechnology, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: kalash0407@mail.ru R.N. Kirakosyan Ph.D. (Biol.), Department of Biotechnology RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: mia41291@mail.ru F.M. Khazieva Ph.D. (Biol.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

The results of cultivation of Potentilla alba L. and Potentilla megalantha L. in vitro are presented. These species are valuable medicinal plants whose cells synthesize different classes of phenolic compounds, in particular tannins with high biological activity. Natural resourcesare not meeting the needs of the pharmaceutical industry. The search for alternative ways of reproduction in vitro of Potentilla plants and preservation of their biodiversity is an urgent task. The aim of this work was to study the reproduction characteristics of Potentilla alba L. and Potentilla megalantha L. in vitro. To achieve this goal, vari-ous methods of pretreatment of seeds were used: mechanical processing, heat treatment, soaking and stratification. As a result, of the conducted research, it was found that when introduced into the culture in vitro Potentilla alba L. and Potentilla megalantha L. it is necessary to apply seed stratification and carry out their germination on a hormone-free medium. Adding BAP to the nutrient medium at the concentra-tion of 1 mg / l leads to the greatest increase in the biomass of P. megalantha and P.alba, as a result of the formation of the maximum number of adventive microbeads.

Keywords: 
Potentilla alba
Potentilla megalantha
in vitro
seed
clonal micropropagation

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