THE INFLUENCE OF ROSE AND EREMOTHECIUM OILS ON THE GROWTH OF SOME LACTIC ACID BACTERIA

DOI: https://doi.org/10.29296/25877313-2018-05-04
Issue: 
5
Year: 
2018

E.F. Semenova Ph.D. (Biol.), Senior Research Scientist, Professor, Medical Institute, Penza State University E-mail: sef1957@mail.ru A.A. Bibarsova Post-graduate Student, Penza State University of Architecture and Construction E-mail: alfiya.bibarsova@mail.ru E.V. Presnyakova Ph.D. (Biol.), Chief Specialist, the State Commission of the Russian Federation on the Use and Protection of Selection Achievements (Moscow) E-mail: spl7@mail.ru

The article is devoted to the investigation of the influence of the essential oils of the Crimean rose and Eremothecium on lactic acid bacteria cultures. The objects under study were collection strains Lactobacillus acidophilus RCAM 01850 and Lactococcus lactisssp. lactis RCAM 02909. The study used samples of the essential oils of the Crimean rose and Eremothecium in serial dilutions in 96% Ethanol 1:250 (I), 1:1000 (II), 1:4000 (III). The content of active substances varied and was 2 μg, 8 μg, and 32 μg per a disk. The analysis of antimicrobial activity of the Rose and Eremothecium oils was carried out by disk-diffusion method (diameter of the disc was 6 mm). The analysis of the results was carried out after 1 ... 7 days by determining the diameter of the delay zones (suppression, inhibition) and the absence (lysed zone) of the culture growth. The influence of different concentrations of aromatic products was determined by comparing with the growth rates of the cultures studied in the presence and absence of essential oils. The experimental material was processed using the Statistica program. The analysis of the effects of essential oils showed that the highest value of the coefficient of variation of the growth retardation zone L. acidophilus under the influence of rose oil was 58.3%, which exceeded the values of the Eremothecium. Eremothecium acted on the culture of L. acidophilus bacteriostatically in all cases. The bactericidal action of Rose oil was detected in 33.3% and bacteriostatic action in 66.7% of cases, which indicates different degrees of antibacterial effects. Both Rose and Eremothecium oils have a bacteriostatic effect but in varying degrees. The variation coefficient of Eremothecium oil antibacterial action was averagely 29,7%, while that of the Rose oil was 17,9%. This is related to the temperate variation of the culture inhibition zones. Thus, the Rose oil revealed significant antimicrobial activity against the culture L. acidophilus, and the Eremothecium one ‒ against the culture L. lactis ssp. lactis. The results of the analysis show the influence of essential oils on the growth of lactic acid bacteria. The data received should be taken into account in the process of optimization of probiotics contents, and the further study of actions of aroma substances and their different concentrations should be continued for the lactic acid bacteria.

Keywords: 
strains of lactic acid bacteria
rose oil
Eremothecium oil
probiotics

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