THE ELLAGITANNINS OF RUBUS MATSUMURANUS: THE COMPONENTIAL COMPOSITION AND PRODUCTION OF UROLITHINS

Raspberry (Rubus L.) is a genus of plants of the cosmopolitan family Rosaceae, common in almost all areas of the globe. Representatives of this family are widely used in officinal and folk medicine in many countries of the world, particularly Rubus matsumuranus H.L.v. & Vaniot. However, according to the literature data on the chemical composition of this species, only three identified compounds is known. The composition of phenolic compounds of R. matsumuranus leaves was investigated. As a result of chromatographic separation eight phenolic compounds were isolated from the leaves of R. matsumuranus: gallic acid, ellagic acid, 2 flavonoids (rutin, isoquercitrin) and 4 ellagitannins (sanguiins H-6 and H-11, lambertianin C, tellimagrandin II). It is known that ellagitannins have low bio-availability and their metabolites are responsible for the manifestation of biological effects in living organisms. The effect of the human intestinal microbiota on the transformation of isolated ellagitannins from R. matsumuranus leaves was studied. HPLC analysis of the sample incubated with human intestinal microbiota revealed the formation of urolithin compounds (dibenzo[b,d]pyran-6-one derivatives) from the ellagitannin fraction. Five metabolites were identified including ellagic acid and urolitins D, C, A and B