THE VARIABILITY OF THE CONTENT OF FLAGOLIGNANS IN FRUITS OF SILYBUM MARIANUM GAERTH. FROM THE NATURAL FLORA OF DAGHESTAN

DOI: https://doi.org/10.29296/25877313-2018-11-03
Issue: 
11
Year: 
2018

F.A. Vagabova Ph.D. (Eng.), Senior Research Scientist, Laboratory of Phytochemistry and Medical Botany, Mountain Botanical Garden, Dagestan Scientific Center of RAS (Makhachkala) Е-mail: fazina@mail.ru M.M. Mamaliyeva Junior Research Scientist, Laboratory of Phytochemistry and Medical Botany, Mountain Botanical Garden, Dagestan Scientific Center of RAS (Makhachkala) Е-mail: mamalieva89@mail.ru A.M. Musaev Senior Research Scientist, a.h. of Laboratory of Phytochemistry and Medical Botany, Mountain Botanical Garden, Dagestan Scientific Center of RAS (Makhachkala) Е-mail: musaev-58@lust.ru A.S. Chubarova Ph.D.(Biol.), Laboratory of Applied Problems of Biology, Biology Faculty, Belarusian State University (Minsk) E-mail: ann.shchekatihina@gmail.com G.K. Radjabov Research Scientist, Laboratory of Phytochemistry and Medical Botany, Mountain Botanical Garden, Dagestan Scientific Center of RAS (Makhachkala) Е-mail: chemfarm@mail.ru

The studying of component content of flavolignan in fruits of Silybum marianum Gaerth. (without coloring of a cover of fruits) from natural Dage-stan samples was the purpose of work. S. marianum grows in Dagestan on dry slopes, fields to the lower mountain belt, along the width gradient, at a continuous strip from the North to the South. The feature of the Dagestan populations of S. marianum is that they consist of plants of winter type, self-pollinators with high intra population variability on coloring of a cover of a fruit - from light-cream to intensively black. Fruits for the analysis were collect-ed in different geographical points of Dagestan in a fruiting stage in June, 2013 and 2015. According to the standard technique with some modification it was received silimarin: the crushed raw materials with a size of particles of 1 mm degreasing petroleum ether, then were extracted by 80% - ethyl alcohol in the ratio 1:1 at 600C in a water bath with the return refrigerator, for the analysis by high performance liquid chromatography method (HLCH) (Agilent Technologies, the USA). The identification of flavolignan in the studied raw materials was carried out on deduction time for HLCH according to internal standards and also with the use of a silibin of Sigma. As standard samples used commercial medicines of a taksifolin (Fluka, Germany), a silimarin and a silibinin (Sigma, the USA) and also the internal standards of a silikristin and silidianin received in laboratory of Applied problems of biology of biological faculty of the Belarusian state university. Silibin and isosilibinin A and isosilibinin B were identified mass spectrometry in BGU laboratory earlier, and for identification in our samples compared them to laboratory standards on keeping time for HLCH. The results of the analysis showed what in the studied samples of S. marianum of strong dispersion by years and in the place of collecting raw materials of the main flavolignan (taksifolin, silikristin, silidianin, silibinin A, silibinin B, isosilibinin A, isosilibinin B) was not observed. The experimental and settlement by methods it is proved that all presented Dagestan samples on the maintenance of flavolignan belong to a si-lidianin hemorasa. It is interesting to note that the studied samples of S. marianum showed low interpopulation differentiation according to contents and composite structure of flavolignan which, perhaps, is connected with lack of intensive microevolutionary processes along the width gradient without existence of ge-ographical barriers.

Keywords: 
milk thistle
Dagestan populations
flavolignans
silibinin
silidianin
chemorase

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